|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||APMA (4-Aminophenylmercuric acetate) activated Human stromelysin-1 (SL-1)|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 2 publications below|
MA5-14199 targets MMP-3 (Stromelysin-1) in IF and WB applications and shows reactivity with Human samples.
The MA5-14199 immunogen is aPMA (4-Aminophenylmercuric acetate) activated Human stromelysin-1 (SL-1).
Matrix metalloproteinases (MMP) are proteolytic enzymes capable of degrading connective tissue components. Imbalanced secretion of certain MMP or disturbances in the differential control of MMP by tissue inhibitor of MMPs (TIMPs) are implicated in the invasive potential of malignant tumors. Stromelysin (MMP3/Transin) degrades several matrix components including the core proteins of proteoglycans, laminin and non-helical regions of collagens.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Blockade of EGFR signaling promotes glioma stem-like cell invasiveness by abolishing ID3-mediated inhibition of p27(KIP1) and MMP3 expression.
MA5-14199 was used in immunohistochemistry to study the roles of ID3, p27(KIP2) and MMP3 in the mechanism by which EGFR inhibition promotes glioma stem cell invasiveness
|Jin X,Jin X,Sohn YW,Yin J,Kim SH,Joshi K,Nam DH,Nakano I,Kim H||Cancer letters (328:235)||2013|
Creation, validation, and quantitative analysis of protein expression in vascular tissue microarrays.
MA5-14199 was used in immunohistochemistry to evaluate the usefulness of tissue microarray technology for protein analysis in vascular segments
|Halushka MK,Cornish TC,Lu J,Selvin S,Selvin E||Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology (19:136)||2010|