Invitrogen

MSH6 Monoclonal Antibody (5B11)

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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MSH6 Antibody in Western Blot (WB) — Western blot was performed using Anti-MSH6 Monoclonal Antibody (5B11) (Product # MA5-15917) and a 160kDa band corresponding to MSH6 was observed in all the tested cell models, except Jurkat and LNCaP which are reported to be negative. This target undergoes heavy phosphorylation and multiple bands between 120-160kDa can be expected. Modified whole cell lysate (1% SDS) (30ug lysate) of K-562 (Lane 1), Jurkat (Lane 2), HeLa (Lane 3), LNCaP (Lane 4), PC-3 (Lane 5), HCT 116 (Lane 6) and A549 (Lane 7) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

MSH6 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of MSH6 was observed in all cell lines and tissue lysates in comparison to Jurkat and LNCaP using MSH6 Monoclonal Antibody (5B11) (Product # MA5-15917) in western blot.. {RE}

Product Details

MA5-15917

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
ELISA (ELISA)	1:10,000	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	5B11
Immunogen	Purified recombinant fragment of human MSH6 expressed in E. Coli.
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	ascites
Contains	0.03% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_11154784

Product Specific Information

MA5-15917 targets MSH6 in WB applications and shows reactivity with Human samples.

The MA5-15917 immunogen is purified recombinant fragment of human MSH6 expressed in E. Coli. .

MA5-15917 detects MSH6 which has a predicted molecular weight of approximately 160kDa.

Target Information

The DNA mismatch repair MSH2/MSH6 heterodimer is responsible for the detection of DNA damage, and may promote apoptosis in normal cells, thus protecting mammals from ultraviolet-induced malignant transformation and hence the prevention of skin cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNA mismatch repair protein Msh6; G/T mismatch-binding protein; GTBP; hMSH6; mutS (E. coli) homolog 6; MutS alpha 160kDa subunit; MutS protein homolog 6; MutS-alpha 160 kDa subunit; MutS-alpha 160kDa subunit; p160; Sperm associated protein; sperm-associated protein

Gene Aliases: GTBP; GTMBP; HNPCC5; HSAP; MSH6; p160

UniProt ID: (Human) P52701

Entrez Gene ID: (Human) 2956

Molecular Function: DNA metabolism protein