Invitrogen

MST1 (STK4) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Reference

View all (22) MST1 (STK4) antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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MST1 (STK4) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of MST1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MST1 Polyclonal Antibody (Product # PA5-53295) at 2 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

MST1 (STK4) Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of MST1 in human lymph node shows strong cytoplasmic positivity in cells outside reaction centra. Samples were probed using a MST1 Polyclonal Antibody (Product # PA5-53295).

MST1 (STK4) Antibody in Western Blot (WB) — Knockout of STK4 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR879788_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of STK4 was performed by loading 30 µg of Caco-2 Wild Type (Lane 1), Caco-2 Cas9 (Lane 2) andCaco-2 STK4 KO (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with a MST1 (STK4) Polyclonal Antibody (Product # PA5-53295, 1:1,000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:5,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to STK4.

MST1 (STK4) Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein inSTK4 KO cell line compared to control cell line using a MST1 (STK4) Polyclonal Antibody (Product # PA5-53295). {KO}

Product Details

PA5-53295

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	View 1 publication 1 publication
Immunohistochemistry (IHC)	1:50-1:200	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human MST1 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2644241

Product Specific Information

Immunogen sequence: IEHDDTLPSQ LGTMVINAED EEEEGTMKRR DETMQPAKPS FLEYFEQKEK ENQINSFGKS VPGPLKNSSD WKIPQDGDYE F

Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 94%.

Target Information

STK4 is a stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. It is a key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation. Phosphorylates MOBKL1A, MOBKL1B and RASSF2. Phosphorylates TNNI3 (cardiac Tn-I) and alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). Phosphorylates FOXO1 on 'Ser-212' and regulates its activation and stimulates transcription of PMAIP1 in a FOXO1-dependent manner. Phosphorylates SIRT1 and inhibits SIRT1-mediated p53/TP53 deacetylation, thereby promoting p53/TP53 dependent transcription and apoptosis upon DNA damage. Acts as an inhibitor of PKB/AKT1. Phosphorylates AR on 'Ser-650' and suppresses its activity by intersecting with PKB/AKT1 signaling and antagonizing formation of AR-chromatin complexes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

LncRNA SNHG17 knockdown promotes Parkin-dependent mitophagy and reduces apoptosis of podocytes through Mst1.

Cell cycle (Georgetown, Tex.)

Guo F,Wang W,Song Y,Wu L,Wang J,Zhao Y,Ma X,Ji H,Liu Y,Li Z,Qin G

PA5-53295 was used in Western Blot to conclude that lncRNA SNHG17 knockdown promotes Parkin-dependent mitophagy and reduces apoptosis of podocytes through regulating the degradation of Mst1.

Sat Aug 01 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: EC 2.7.11.1; kinase MST1; kinase responsive to stress 2; Mammalian STE20-like protein kinase 1; mammalian sterile 20-like 1; MST-1; MST1/C; MST1/N; Serine/threonine-protein kinase 4; Serine/threonine-protein kinase 4 18kDa subunit; Serine/threonine-protein kinase 4 37kDa subunit; Serine/threonine-protein kinase Krs-2; STE20-like kinase MST1; sterile 20-like kinase 1; TIIAC; Yeast Sps1/Ste20-related kinase 3

Gene Aliases: AI447339; AU020804; Kas-2; KRS2; MST1; STK4; YSK3

UniProt ID: (Human) Q13043, (Mouse) Q9JI11

Entrez Gene ID: (Human) 6789, (Rat) 311622, (Mouse) 58231

Molecular Function: non-receptor serine/threonine protein kinase