|Tested species reactivity||Mouse|
|Host / Isotype||Horse / IgG|
|Storage buffer||PBS, pH 7.8, with sucrose|
|Contains||0.08% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product # 31181 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31181 reacts with heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins. Based on solid-phase enzyme immunoassay, this antibody cross-reacts with rat IgG (50%), hamster IgG and mouse IgM (25%), hamster IgM (6%), guinea pig IgG and swine IgG (1%). It has minimum cross-reactivity with immunoglobulins from other species.
Store lyophilized product at 4°C until opened. Restore with 0.5 ml distilled water (3.0 mg/ml after restoration) and undiluted liquid may be stored for several weeks at 4°C. To extend the shelf-life of this product, freeze undiluted product in small aliquots. Avoid repeated freezing and thawing.
Thermo Scientific Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.