|Dot blot (DB)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:200-1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:1000|
|Immunomicroscopy (IM)||Assay dependent|
|Western Blot (WB)||1:500-1:2,500|
|Tested Species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Full length native Mouse IgG (purified).|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/ml BSA|
|Contains||0.01% gentamicin sulfate|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
PA1-28761 detects Mouse IgG from mouse samples. Minimum cross reactivity to human serum proteins.
PA1-28761 has been successfully used in ELISA, immunohistochemistry (frozen tissue), Immunomicroscopy, Western blot, immunohistochemistry (paraffin tissue) and dot blot applications.
The PA1-28761 immunogen is full length native Mouse IgG (purified).
Thermo Scientific Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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