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Product Details
MA5-18093
| Applications | Tested Dilution |
|---|---|
Flow Cytometry (Flow) |
10 µLx10^6 cells or 100 µL whole blood |
Control (Ctrl) |
Assay-Dependent |
| Product Specifications | |
|---|---|
Host/Isotype |
Mouse / IgG1 |
Class |
Control |
Type |
Isotype Control |
Immunogen |
Rat thymocyte membrane glycoproteins |
Conjugate |
APC |
Excitation/Emission Max |
651/660 nm
View spectra
|
Form |
Lyophilized |
Concentration |
Conc. Not Determined |
Purification |
Protein A |
Storage buffer |
PBS with 1% BSA, 5% sucrose |
Contains |
0.09% sodium azide |
Storage conditions |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_2539476 |
Product Specific Information
MA5-18093 is negative on all human cells and cell lines tested. This antibody recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species. Test results have shown that MA5-18093 is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.
Reconstitute with 1.0 mL of distilled water. After reconstitution, store product undiluted at 4°C in the dark.
Target Information
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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