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Invitrogen
Mouse IgG2b Isotype Control, Pacific Blue
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Product Details
MG2B28
Applications
Tested Dilution
Control (Ctrl)
Assay-Dependent
Flow Cytometry (Flow)
Assay-Dependent
Product Specifications
Host/Isotype
Mouse
/ IgG2b
Class
Control
Type
Isotype Control
Immunogen
Mouse IgG2b
Conjugate
Excitation/Emission Max
Form
Liquid
Concentration
100 µg/mL
Purification
purified
Storage buffer
PBS with BSA
Contains
0.1% sodium azide
Storage conditions
4° C, store in dark
Shipping conditions
Blue
RRID
Target Information
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgG2; IgG2b
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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