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Product Details
PA5-33225
Applications
Tested Dilution
Flow Cytometry (Flow)
Neat- 1/10
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Isotype Control
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2550640
Product Specific Information
PA5-33225 is a mouse IgG2b monoclonal antibody arising from a spontaneous mouse plasmacytoma. The antibody does not react with any human cell surface markers, and may therefore be used as an isotype matched negative control reagent to assess the level of non-specific binding of mouse IgG2b monoclonal antibodies in human cells.
PA5-33225 is also suitable for use as a negative control with bovine, sheep, pig, canine, rat and guinea-pig tissues.
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse IgG2b Negative Control is negative on all human cells and cell lines tested. This antibody recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species. Test results show that MCA691C is also suitable for use as a negative control with bovine, ovine, porcine, canine and guinea-pig tissues.
Target Information
Mouse IgG2b is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the third most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2b is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2b is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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