|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against mouse IgM, mouse IgA, pooled human sera, purified human paraproteins and mouse isotypes IgG1, IgG2a and IgG3 prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 4 publications below|
Flourescence of this long-wavelength Alexa Fluor dye is not visible by looking through a conventional fluorescence microscope.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Association of TMEM16A chloride channel overexpression with airway goblet cell metaplasia.||Scudieri P,Caci E,Bruno S,Ferrera L,Schiavon M,Sondo E,Tomati V,Gianotti A,Zegarra-Moran O,Pedemonte N,Rea F,Ravazzolo R,Galietta LJ||The Journal of physiology (590:6141)||2012|
|Not Applicable||Not Cited||A guide to accurate fluorescence microscopy colocalization measurements.||Comeau JW,Costantino S,Wiseman PW||Biophysical journal (91:4611)||2006|
|Not Applicable||Not Cited||ASC-mediated NF-kappaB activation leading to interleukin-8 production requires caspase-8 and is inhibited by CLARP.||Hasegawa M,Imamura R,Kinoshita T,Matsumoto N,Masumoto J,Inohara N,Suda T||The Journal of biological chemistry (280:15122)||2005|
|Not Applicable||Not Cited||Applicability of laser scanning cytometry to study paediatric alveolar macrophages.||Bunn HJ,Woltmann G,Grigg J||The European respiratory journal (20:1437)||2002|