Immunohistochemistry analysis of Mucin 1 / MUC-1 (E29) showing staining in the membrane and weak cytoplasm of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Mucin 1 / MUC-1 Antibody (E29) Mouse Monoclonal Antibody (MA514077) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Delipidated extract of human milk fat globule membranes|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14077 targets Epithelial Membrane Antigen in IHC (P) applications and shows reactivity with Human samples.
The MA5-14077 immunogen is delipidated extract of human milk fat globule membranes.
Epithelial membrane antigen (EMA), is a mucin-like glycoprotein. Antibody to EMA has been shown useful as a pan- epithelial marker for detecting early metastatic loci of carcinoma in the bone marrow or liver.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Primary acinic cell carcinoma of the breast: a case report and review of the literature.
MA5-14077 was used in immunohistochemistry to report the case of primary acinic cell carcinoma of the breast in a 38-year-old woman.
|Zhao Y,Li W,Lang R,Yang Y,Gao X,Zheng Y,Zhang C,Fu X,Fu L||International journal of surgical pathology (22:177)||2014|
Pancreatic mucinous cystic tumor in Turner syndrome: How a tumor bends to a genetic disease.
MA5-14077 was used in immunohistochemistry to report on a case of pancreatic mucinous cystic tumor in a patient with Turner syndrome
|Pizzi M,Pennelli G,Merante-Boschin I,Fassan M,Pelizzo MR,Rugge M||International journal of surgery case reports (4:1028)||2013|
An immunohistochemical study of the origin of the solid strand in syringoma, using carcinoembryonic antigen, epithelial membrane antigen, and cytokeratin 5.
MA5-14077 was used in immunohistochemistry to study the precise cellular origin of syringoma
|Kim BC,Park EJ,Kwon IH,Cho HJ,Park HR,Kim KH,Kim KJ||International journal of dermatology (51:817)||2012|
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
MA5-14077 was used in immunohistochemistry to report on a case of sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression
|Yin WH,Li J,Chan JK||Diagnostic pathology (7:null)||2012|
Anaplastic large cell lymphoma with paraneoplastic leukocytosis: a clinicopathological analysis of five cases.
MA5-14077 was used in immunohistochemistry to report on five cases of anaplastic large cell lymphoma with paraneoplastic leukocytosis
|Chang IW,Chen HK,Ma MC,Huang WT||APMIS : acta pathologica, microbiologica, et immunologica Scandinavica (119:794)||2011|
Atypical teratoid/rhabdoid tumors may show morphological and immunohistochemical features seen in choroid plexus tumors.
MA5-14077 was used in immunohistochemistry to study the morphological and immunohistochemical features of atypical teratoid/rhabdoid tumors
|Schittenhelm J,Nagel C,Meyermann R,Beschorner R||Neuropathology : official journal of the Japanese Society of Neuropathology (31:461)||2011|
Infratentorial ependymomas: prognostic factors and outcome analysis in a multi-center retrospective series of 106 adult patients.
MA5-14077 was used in immunohistochemistry to evaluate the major prognostic factors for posterior fossa ependymomas
|Guyotat J,Metellus P,Giorgi R,Barrie M,Jouvet A,Fevre-Montange M,Chinot O,Durand A,Figarella-Branger D||Acta neurochirurgica (151:947)||2009|
Primary choroid plexus papilloma of the sacral nerve roots.
MA5-14077 was used in immunohistochemistry to report on a case of choroid plexus papilloma of the sacral nerve roots
|Boldorini R,Panzarasa G,Girardi P,Monga G||Journal of neurosurgery. Spine (10:51)||2009|
Dedifferentiated Liposarcoma of the Retroperitoneum with Extensive Leiomyosarcomatous Differentiation and beta-Human Chorionic Gonadotropin Production.
MA5-14077 was used in immunohistochemistry to report a clinical case of dedifferentiated liposarcoma of the retroperitoneum with unique features
|Russell MJ,Flynt FL,Harroff AL,Fadare O||Sarcoma (2008:null)||2008|
An immunohistochemical profile of giant cell carcinoma of the larynx.
MA5-14077 was used in immunohistochemistry to report on a case of giant cell carcinoma of the larynx
|Gurbuz Y,Kose N,Aydin O,Ozturk M||Auris, nasus, larynx (34:413)||2007|
Recurrent solitary fibrous tumour in the cerebellopontine angle.
MA5-14077 was used in immunohistochemistry to report a clinical case of solitary fibrous tumour in the cerebellopontine angle
|Bikmaz K,Cosar M,Kurtkaya-Yapicier O,Iplikcioglu AC,Gokduman CA||Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia (12:829)||2005|
Collagenous fibroma (desmoplastic fibroblastoma).
MA5-14077 was used in immunohistochemistry to report on a patient with collagenous fibroma
|Dagli M,Eryilmaz A,Acar A,Kulacoglu S,Akmansu H||Yonsei medical journal (45:941)||2004|
A confirmatory report for the close interaction of Helicobacter pylori with gastric epithelial MUC5AC expression.
MA5-14077 was used in immunohistochemistry to study the effect of Helicobacter pylori on gastric mucin expression
|Kocer B,Ulas M,Ustundag Y,Erdogan S,Karabeyoglu M,Yldrm O,Unal B,Cengiz O,Soran A||Journal of clinical gastroenterology (38:496)||2004|
Prognostic significance of mucin expression in gastric carcinoma.
MA5-14077 was used in immunohistochemistry to investigate the prognostic value of mucin in the gastric carcinoma
|Kocer B,Soran A,Kiyak G,Erdogan S,Eroglu A,Bozkurt B,Solak C,Cengiz O||Digestive diseases and sciences (49:954)||2004|
Diagnostic and therapeutic strategy and the most efficient prognostic factors of breast malignant fibrous histiocytoma.
MA5-14077 was used in immunohistochemistry - paraffin section to report the clinicopathological features of 9 breast malignant fibrous histiocytoma patients
|Qiu SQ,Wei XL,Huang WH,Wu MY,Qin YS,Li YK,Zhang GJ||Scientific reports (3:null)||2013|
Anti-tumor effect of the anti-KL-6/MUC1 monoclonal antibody through exposure of surface molecules by MUC1 capping.
MA5-14077 was used in blocking or activating experiment to study the mechanism underlying the anti-tumor effects of an anti-KL-6/MUC1 monoclonal antibody
|Doi M,Yokoyama A,Kondo K,Ohnishi H,Ishikawa N,Hattori N,Kohno N||Cancer science (97:420)||2006|