Immunofluorescence analysis of MyoD1 was done on 70% confluent log phase C2C12 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MyoD1 (5.8A) Mouse Monoclonal Antibody (MA512902) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Chicken, Human, Mouse, Rat|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant mouse MyoD1 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2-3 µg/mL|
|Immunofluorescence (IF)||2-3 µg/mL|
|Immunomicroscopy (IM)||Assay Dependent|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||2-3 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12902 targets MyoD1 in IF, IM, and IP applications and shows reactivity with Chicken, Human, mouse, and Rat samples.
The MA5-12902 immunogen is recombinant mouse MyoD1 protein.
MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occassionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm's tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing's sarcomas and alveolar soft part sarcomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Genetic disruption of Smad7 impairs skeletal muscle growth and regeneration.
MA5-12902 was used in immunohistochemistry - frozen section to elucidate the role of SMAD7 in muscle
|Cohen TV,Kollias HD,Liu N,Ward CW,Wagner KR||The Journal of physiology (593:2479)||2015|
Molecular characterization of an EWSR1-POU5F1 fusion associated with a t(6;22) in an undifferentiated soft tissue sarcoma.
MA5-12902 was used in immunohistochemistry to characterize a clinical case of undifferentiated soft tissue sarcoma with chromosomal rearrangement and resultant EWSR1-POU5F1 fusion
|Deng FM,Galvan K,de la Roza G,Zhang S,Souid AK,Stein CK||Cancer genetics (204:423)||2011|
CD90-positive cells, an additional cell population, produce laminin alpha2 upon transplantation to dy(3k)/dy(3k) mice.
MA5-12902 was used in immunocytochemistry to study the role of CD90-positive cells in producing laminin alpha2 upon transplantation to dy(3k)/dy(3k) mice
|Fukada S,Yamamoto Y,Segawa M,Sakamoto K,Nakajima M,Sato M,Morikawa D,Uezumi A,Miyagoe-Suzuki Y,Takeda S,Tsujikawa K,Yamamoto H||Experimental cell research (314:193)||2008|
Molecular signature of quiescent satellite cells in adult skeletal muscle.
MA5-12902 was used in immunocytochemistry to investigate gene expression in quiescent satellite cells of adult skeletal muscle
|Fukada S,Uezumi A,Ikemoto M,Masuda S,Segawa M,Tanimura N,Yamamoto H,Miyagoe-Suzuki Y,Takeda S||Stem cells (Dayton, Ohio) (25:2448)||2007|
ADP-ribosylation of integrin alpha7 modulates the binding of integrin alpha7beta1 to laminin.
MA5-12902 was used in western blot to study the effect of ADP-ribosylation on the interaction between integrin alpha7beta1 and laminin
|Zhao Z,Gruszczynska-Biegala J,Zolkiewska A||The Biochemical journal (385:309)||2005|
Roles of peroxisome proliferator-activated receptors delta and gamma in myoblast transdifferentiation.
MA5-12902 was used in western blot to study the roles of PPARdelta and gamma in myoblast transdifferentiation
|Holst D,Luquet S,Kristiansen K,Grimaldi PA||Experimental cell research (288:168)||2003|