|Tested species reactivity||Bovine, Chicken, Human, Pig, Rabbit|
|Host / Isotype||Mouse / IgM|
|Immunogen||Chicken lens membranes.|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-24940 detects Myosin light chain from human, bovine, chicken, porcine, and rabbit samples.
MA1-24940 has been successfully used in Western blot and immunofluorescence procedures.
The MA1-24940 immunogen is chicken lens membranes.
Contractile proteins are ubiquitous in eukaryotic cells. Their presence is related to motile functions. The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Numerous investigators have shown that actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. Myosin is a 500,000 dalton protein known to interact with identical heavy chains (200,000 daltons each) and four light chains (15,000-20,000 daltons). Myosin molecules consist of two major regions: tail (rod) and head regions. Myosin has three major biological functions:1. Myosin molecules spontaneously assemble into filaments in solutions of physiologic ionic strength and pH. Thick filaments consist mainly of myosin molecules.2. Myosin is an enzyme (ATPase) and its activity is the immediate source of the free energy that drives muscle contraction.3. Myosin binds to the polymerized form of actin, the major constituent of the thin filament.Myosin can be viewed in immunofluorescent microscopy enabling the delineation of the intracellular organization of proteins that are in the form of a supramolecular structure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.