|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from internal domain of human NFκB p65.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is prostate or placenta tissue.
NFκB is a heterodimer consisting of a 50kDa DNA binding subunit and a 65kDa transactivating subunit (RelA). The p50/p65 heterodime is present in the cytoplasm in inactive form bound to its inhibitor, IκB. In unstimulated cells, nuclear localization signals present on p65 are masked by members of the IκB family of inhibitory proteins. Subsequent to cell stimulation, IκB undergoes phosphorylation, ubiquitination and degradation by a proteosome-dependent pathway, allowing nuclear translocation of the active dimeric NFκB transcription factor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.