Immunofluorescent analysis of NIK in NIK-transfected 293 cells (right) and untransfected cells (left) using a NIK recombinant rabbit monoclonal antibody (Product # 700281) at a dilution of 5ug/ml followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000 (green) and nuclei stained using Hoescht (blue).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A recombinant protein corresponding to amino acids 706-860 of Q99558.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||4-6 ug/ml|
|Immunofluorescence (IF)||4-6 ug/ml|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with Rhesus monkey, mouse, rat, bovine, equine and chicken based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
NIK, also known as mitogen-activated protein kinase kinase kinase (MAP3K) 14 is an NF-kappaB inducing protein kinase of the serine/threonine kinase family. NIK binds to Traf2 to activate NF-kappaB as part of a signaling cascade involving receptors of the TNF/NGF family and the interleukin-1 type-I receptor. NIK protein is shuttled between cytosolic, nuclear and nucleolar compartments. When shuttled to the nucleoli, NIK-dependant NF-kappaB activation is impaired relative to its nuclear localization revealing a possible means of regulation. Reactive oxygen species can interact directly with NIK to alter NF-kappaB activation. Additionally, NIK specifically interacts with Down syndrome candidate region 1 (DSCR1) blocking its proteosomal degradation leading to DSCR1 accumulation and cytotoxicity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.