Invitrogen

NK1.1 Monoclonal Antibody (PK136), APC-eFluor™ 780, eBioscience™

Product Image Gallery

NK1.1 Antibody in Flow Cytometry (Flow) — Staining of C57Bl/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) eFluor® 450 (Product # 48-5971-82) and 0.5 µg of Mouse IgG2a K Isotype Control APC-eFluor® 780 (Product # 47-4724) (left) or 0.5 µg of Anti-Mouse NK1-1 APC-eFluor® 780 (right). Total viable cells were used for analysis.

Product Details

47-5941-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	1 µg/test	View 32 publications 32 publications

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Mouse / IgG2a, kappa
Class	Monoclonal
Type	Antibody
Clone	PK136
Conjugate	APC-eFluor™ 780 Alexa Fluor 561 Alexa Fluor 660 Alexa Fluor 700 APC Biotin Brilliant UV 395 Brilliant UV 496 Brilliant UV 661 Brilliant UV 805 Brilliant Violet 421 Brilliant Violet 480 Brilliant Violet 650 Brilliant Violet 711 Brilliant Violet 786 eFluor 450 FITC Functional Grade PE PE-Cyanine7 PE-eFluor 610 PerCP-Cyanine5.5 Super Bright 436 Super Bright 600 Super Bright 645 Super Bright 702
Excitation/Emission Max	756/785 nm View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2735070

Product Specific Information

Description: The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. Several commonly used laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H and A do not express the NK1.1 antigen. For detection of NK cells in these strains the monoclonal antibody DX5 (Cat. No. 14-5971) should be used. Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells.

Applications Reported: This PK136 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This PK136 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

NK1.1 (Killer cell lectin-like receptor subfamily B, member 1, KLRB1, NKR-P1A, CD161, cluster of differentiation 161), refers to Natural Killer (NK) cells, lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein, NKR-P1A or CD161, is classified as a type II membrane protein because it has an external C terminus. NKR-P1A, the receptor encoded by the KLRB1 gene, recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

NK cells require immune checkpoint receptor LILRB4/gp49B to control neurotropic Zika virus infections in mice.

JCI insight

Lee HN,Manangeeswaran M,Lewkowicz AP,Engel K,Chowdhury M,Garige M,Eckhaus MA,Sourbier C,Ireland DD,Verthelyi D

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Tue Feb 08 00:00:00 EST 2022

Exosomal lncRNA SNHG10 derived from colorectal cancer cells suppresses natural killer cell cytotoxicity by upregulating INHBC.

Cancer cell international

Huang Y,Luo Y,Ou W,Wang Y,Dong D,Peng X,Luo Y

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

Expression of CCRL2 Inhibits Tumor Growth by Concentrating Chemerin and Inhibiting Neoangiogenesis.

Cancers

Al Delbany D,Robert V,Dubois-Vedrenne I,Del Prete A,Vernimmen M,Radi A,Lefort A,Libert F,Wittamer V,Sozzani S,Parmentier M

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Tue Oct 05 00:00:00 EDT 2021

A novel miR1983-TLR7-IFNβ circuit licenses NK cells to kill glioma cells, and is under the control of galectin-1.

Oncoimmunology

Shah D,Comba A,Faisal SM,Kadiyala P,Baker GJ,Alghamri MS,Doherty R,Zamler D,Nuñez G,Castro MG,Lowenstein PR

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

Sequencing, cloning, and antigen binding analysis of monoclonal antibodies isolated from single mouse B cells.

STAR protocols

Viant C,Escolano A,Chen ST,Nussenzweig MC

47-5941-82 was used in Flow Cytometry to analyze the BCRs of different mouse B cell populations for somatic hypermutations, clonal and phylogenic relationships, and their affinity for cognate antigen.

Fri Jun 18 00:00:00 EDT 2021

The transcriptional repressor ID2 supports natural killer cell maturation by controlling TCF1 amplitude.

The Journal of experimental medicine

Li ZY,Morman RE,Hegermiller E,Sun M,Bartom ET,Maienschein-Cline M,Sigvardsson M,Kee BL

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Mon Jun 07 00:00:00 EDT 2021

Near-infrared photoimmunotherapy targeting human-EGFR in a mouse tumor model simulating current and future clinical trials.

EBioMedicine

Okada R,Furusawa A,Vermeer DW,Inagaki F,Wakiyama H,Kato T,Nagaya T,Choyke PL,Spanos WC,Allen CT,Kobayashi H

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Conventional NK cells and ILC1 are partially ablated in the livers of Ncr1 ^iCreTbx21 ^fl/fl mice.

Wellcome open research

Cuff AO,Male V

47-5941 was used in Flow cytometry/Cell sorting to examine the role of Tbet-dependent type I innate lymphoid cells in the murine liver.

Sat Jan 09 00:00:00 EST 2021

B7-CD28 co-stimulation modulates central tolerance via thymic clonal deletion and Treg generation through distinct mechanisms.

Nature communications

Watanabe M,Lu Y,Breen M,Hodes RJ

47-5941 was used in Flow cytometry/Cell sorting to reveal a function of B7-CD28 co-stimulation in shaping the T cell repertoire and limiting autoimmunity through both thymic clonal deletion and Treg cell generation.

Tue Dec 08 00:00:00 EST 2020

The Obese Liver Environment Mediates Conversion of NK Cells to a Less Cytotoxic ILC1-Like Phenotype.

Frontiers in immunology

Cuff AO,Sillito F,Dertschnig S,Hall A,Luong TV,Chakraverty R,Male V

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Wed Oct 21 00:00:00 EDT 2020

IL-2/Anti-IL-2 Complex Attenuates Inflammation and BBB Disruption in Mice Subjected to Traumatic Brain Injury.

Frontiers in neurology

Gao W,Li F,Zhou Z,Xu X,Wu Y,Zhou S,Yin D,Sun D,Xiong J,Jiang R,Zhang J

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Diversity in medullary thymic epithelial cells controls the activity and availability of iNKT cells.

Nature communications

Lucas B,White AJ,Cosway EJ,Parnell SM,James KD,Jones ND,Ohigashi I,Takahama Y,Jenkinson WE,Anderson G

47-5941 was used in Flow cytometry/Cell sorting to study the mechanism that controls the development of αβ T cell lineages in the thymus.

Mon May 04 00:00:00 EDT 2020

Synergized regulation of NK cell education by NKG2A and specific Ly49 family members.

Nature communications

Zhang X,Feng J,Chen S,Yang H,Dong Z

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

Antitumor immunity augments the therapeutic effects of p53 activation on acute myeloid leukemia.

Nature communications

Hayashi Y,Goyama S,Liu X,Tamura M,Asada S,Tanaka Y,Fukuyama T,Wunderlich M,O'Brien E,Mizukawa B,Yamazaki S,Matsumoto A,Yamasaki S,Shibata T,Matsuda K,Sashida G,Takizawa H,Kitamura T

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Fri Oct 25 00:00:00 EDT 2019

Progranulin prevents regulatory NK cell cytotoxicity against antiviral T cells.

JCI insight

Huang A,Shinde PV,Huang J,Senff T,Xu HC,Margotta C,Häussinger D,Willnow TE,Zhang J,Pandyra AA,Timm J,Weggen S,Lang KS,Lang PA

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Thu Sep 05 00:00:00 EDT 2019

Characterization of Transcriptional Regulatory Networks that Promote and Restrict Identities and Functions of Intestinal Innate Lymphoid Cells.

Immunity

Pokrovskii M,Hall JA,Ochayon DE,Yi R,Chaimowitz NS,Seelamneni H,Carriero N,Watters A,Waggoner SN,Littman DR,Bonneau R,Miraldi ER

47-5941 was used in Flow cytometry/Cell sorting to study regulatory interactions between transcription factors and genes within intestinal type 1, 2, and 3 innate lymphoid cells subsets.

Tue Jul 16 00:00:00 EDT 2019

Immunization expands B cells specific to HIV-1 V3 glycan in mice and macaques.

Nature

Escolano A,Gristick HB,Abernathy ME,Merkenschlager J,Gautam R,Oliveira TY,Pai J,West AP,Barnes CO,Cohen AA,Wang H,Golijanin J,Yost D,Keeffe JR,Wang Z,Zhao P,Yao KH,Bauer J,Nogueira L,Gao H,Voll AV,Montefiori DC,Seaman MS,Gazumyan A,Silva M,McGuire AT,Stamatatos L,Irvine DJ,Wells L,Martin MA,Bjorkman PJ,Nussenzweig MC

47-5941-82 was used in Flow Cytometry to develop an immunogen, RC1, that facilitates the recognition of the variable loop 3 (V3)-glycan patch on the envelope protein of HIV-1 to activate B cells that express precursors of broadly neutralizing antibodies within polyclonal repertoires.

Sat Jun 01 00:00:00 EDT 2019

Immunization expands B cells specific to HIV-1 V3 glycan in mice and macaques.

Nature

Sat Jun 01 00:00:00 EDT 2019

Plasmodium co-infection protects against chikungunya virus-induced pathologies.

Nature communications

Teo TH,Lum FM,Ghaffar K,Chan YH,Amrun SN,Tan JJL,Lee CYP,Chua TK,Carissimo G,Lee WWL,Claser C,Rajarethinam R,Rénia L,Ng LFP

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Tue Sep 25 00:00:00 EDT 2018

CD8⁺ T cells mediate the antitumor activity of frankincense and myrrh in hepatocellular carcinoma.

Journal of translational medicine

Xu C,Lu X,Liu W,Chen A,Meng G,Zhang H,Li B,Zhang Y,Wu J,Wei J

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Mon May 21 00:00:00 EDT 2018

Loss of HIF-1α in natural killer cells inhibits tumour growth by stimulating non-productive angiogenesis.

Nature communications

Krzywinska E,Kantari-Mimoun C,Kerdiles Y,Sobecki M,Isagawa T,Gotthardt D,Castells M,Haubold J,Millien C,Viel T,Tavitian B,Takeda N,Fandrey J,Vivier E,Sexl V,Stockmann C

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Fri Nov 17 00:00:00 EST 2017

A TLR7 agonist enhances the antitumor efficacy of obinutuzumab in murine lymphoma models via NK cells and CD4 T cells.

Leukemia

Cheadle EJ,Lipowska-Bhalla G,Dovedi SJ,Fagnano E,Klein C,Honeychurch J,Illidge TM

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2017

Differentiation of human and murine induced pluripotent stem cells to microglia-like cells.

Nature neuroscience

Pandya H,Shen MJ,Ichikawa DM,Sedlock AB,Choi Y,Johnson KR,Kim G,Brown MA,Elkahloun AG,Maric D,Sweeney CL,Gossa S,Malech HL,McGavern DB,Park JK

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

Dendritic cells, macrophages, NK and CD8⁺ T lymphocytes play pivotal roles in controlling HSV-1 in the trigeminal ganglia by producing IL1-beta, iNOS and granzyme B.

Virology journal

Lucinda N,Figueiredo MM,Pessoa NL,Santos BS,Lima GK,Freitas AM,Machado AM,Kroon EG,Antonelli LR,Campos MA

47-5941 was used in Flow cytometry/Cell sorting to provide strong evidence that the responses mediated by DCs, Mo/M?, NK and CD8+ T lymphocytes through IL-1β, iNOS and granzyme B production, respectively, together with the production of type I IFN early in the infection, are crucial to host defense against HSV-1.

Tue Feb 21 00:00:00 EST 2017

NK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation.

Nature communications

Yang M,Chen S,Du J,He J,Wang Y,Li Z,Liu G,Peng W,Zeng X,Li D,Xu P,Guo W,Chang Z,Wang S,Tian Z,Dong Z

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Wed Sep 07 00:00:00 EDT 2016

The breast tumor microenvironment alters the phenotype and function of natural killer cells.

Cellular & molecular immunology

Krneta T,Gillgrass A,Chew M,Ashkar AA

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

Favorable alteration of tumor microenvironment by immunomodulatory cytokines for efficient T-cell therapy in solid tumors.

PloS one

Tähtinen S,Kaikkonen S,Merisalo-Soikkeli M,Grönberg-Vähä-Koskela S,Kanerva A,Parviainen S,Vähä-Koskela M,Hemminki A

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Thu Apr 28 00:00:00 EDT 2016

Hypoxia-Inducible Factor-2α Limits Natural Killer T Cell Cytotoxicity in Renal Ischemia/Reperfusion Injury.

Journal of the American Society of Nephrology : JASN

Zhang J,Han C,Dai H,Hou J,Dong Y,Cui X,Xu L,Zhang M,Xia Q

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Leukocyte population dynamics and detection of IL-9 as a major cytokine at the mouse fetal-maternal interface.

PloS one

Habbeddine M,Verbeke P,Karaz S,Bobé P,Kanellopoulos-Langevin C

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Wed Jun 10 00:00:00 EDT 2015

Metronomic cyclophosphamide eradicates large implanted GL261 gliomas by activating antitumor Cd8⁺ T-cell responses and immune memory.

Oncoimmunology

Wu J,Waxman DJ

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Antigen delivery to CD11c+CD8- dendritic cells induces protective immune responses against experimental melanoma in mice in vivo.

Journal of immunology (Baltimore, Md. : 1950)

Neubert K,Lehmann CH,Heger L,Baranska A,Staedtler AM,Buchholz VR,Yamazaki S,Heidkamp GF,Eissing N,Zebroski H,Nussenzweig MC,Nimmerjahn F,Dudziak D

47-5941 was used in Flow cytometry/Cell sorting to assess the anti-tumour efficacy of a highly selective immunisation-based therapy.

Sun Jun 15 00:00:00 EDT 2014

Lymphoma-like T cell infiltration in liver is associated with increased copy number of dominant negative form of TGFβ receptor II.

PloS one

Zhang W,Tsuda M,Yang GX,Tsuneyama K,He XS,Ansari AA,Ridgway WM,Coppel RL,Lian ZX,Leung PS,Gershwin ME

47-5941 was used in Flow cytometry/Cell sorting to further define the processes underlying the development of lymphoma-like T cell infiltration.

Tue Jun 25 00:00:00 EDT 2013

VEGF receptor inhibitors block the ability of metronomically dosed cyclophosphamide to activate innate immunity-induced tumor regression.

Cancer research

Doloff JC,Waxman DJ

Published figure using NK1.1 monoclonal antibody (Product # 47-5941-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: CD161 antigen-like family member B; CD161 antigen-like family member C; CD161b; CD161c; Inhibitory receptor NKR-P1B; killer cell lectin-like receptor subfamily A member 1C; killer cell lectin-like receptor subfamily B member 1B allele A; Killer cell lectin-like receptor subfamily B member 1B allele B; Killer cell lectin-like receptor subfamily B member 1C; killer cell lectin-like receptor subfamily B member 1D; KLRB1; Ly-55; ly-55b; Ly-55c; Ly-55d; Ly55; lymphocyte antigen 55 complex, locus B; lymphocyte antigen 55 complex, locus C; lymphocyte antigen 55 complex, locus D; lymphocyte antigen 55b; Lymphocyte antigen 55c; Lymphocyte antigen 55d; lymphocyte antigen 59; MGC163757; MGC163759; natural killer cell receptor protein NKR-P1B; natural killer cell receptor protein NKR-P1C; natural killer cell receptor-P1; Natural killer cell surface protein NKR-P1B allele B6; natural killer cell surface protein NKR-P1B allele SJL/BALB; Natural killer cell surface protein P1-40; natural killer cell-associated antigen 1; natural killer cells; nk cells; NK1.1; NKR-P1 34; NKR-P1 40; NKR-P1.9; NKR-P1C; NKR-P1D

Gene Aliases: AI462337; CD161; Klrb1b; Klrb1c; Klrb1d; ly-55c; Ly-59; Ly55b; Ly55c; Ly55d; Ly59; Nk-1; Nk-1.2; NK-RP1; Nk1; NK1.1; Nk1.2; NKR-P1.9; NKR-P1B; NKR-P1D; NKRP1; Nkrp1-b; NKRP140; Nkrp1b; Nkrp1c; Nkrp1d

UniProt ID: (Mouse) Q99JB4, (Mouse) P27814

Entrez Gene ID: (Mouse) 80782, (Mouse) 17059

Molecular Function: transmembrane signal receptor