OriGene

NRBP1 Monoclonal Antibody (OTI4C1), TrueMAB™

Product Image Gallery

NRBP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human Kidney tissue within the normal limits using anti-NRBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500461)

NRBP1 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NRBP1 (RC200107, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-NRBP1. Positive lysates LY415614 (100 µg) and LC415614 (20 µg) can be purchased separately from OriGene.

NRBP1 Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC200107 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NRBP1 antibody (TA500461), and then analyzed by flow cytometry.

NRBP1 Antibody in Immunoprecipitation (IP) — Immunoprecipitation(IP) of NRBP1 by using TrueMab monoclonal anti-NRBP1 antibodies (Negative control: IP without adding anti-NRBP1 antibody.). For each experiment, 500ul of tagged NRBP1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 µg of anti-NRBP1 antibody and 20ul (0.1mg) of goat anti-mouse conj µgated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Product Details

CF500461

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50	-
Flow Cytometry (Flow)	1:100	-
Immunoprecipitation (IP)	2 µg/500 µL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Clone	OTI4C1
Immunogen	Full-length protein expressed in 293T cell transfected with human NRBP1 expression vector View antigen
Conjugate	Unconjugated
Form	lyophilized
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.3, with 8% trehalose
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases. Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the intracellular membrane structure associated with flaviviral replication. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.