|Tested species reactivity||Bovine, Chicken, Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human NSF.|
|Storage buffer||PBS, pH 7.5, with 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
A suggested positive control for this product is PC12 cells.
NSF (N-ethylmaleimide sensitive fusion protein) is a critical component of the SNARE (soluble NSF attachment protein receptors) protein complex that is involved in synaptic vesicle trafficking. Specifically, NSF has been found to be essential in membrane fusion. Furthermore, NSF has been recently demonstrated to bind other protein complexes such as AMPA receptor subunits (GluR2), GATE-16, LMA-1 and Rabs suggesting a more diverse role in the assembly of various protein complexes (Whiteheart et al., 2004).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The schizophrenia risk gene product miR-137 alters presynaptic plasticity.
MA1-12435 was used in western blot to characterize induced human neurons harboring alleles of four disease-associated single nucleotide polymorphisms in MIR137
|Siegert S,Seo J,Kwon EJ,Rudenko A,Cho S,Wang W,Flood Z,Martorell AJ,Ericsson M,Mungenast AE,Tsai LH||Nature neuroscience (18:1008)||2015|