|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Insoluble NFT which were extracted in boiling sodium dodecyl sulfate, purified by sucrose gradient centrifugation and disaggregated by sonication.|
|Storage buffer||whole serum|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Functional Assay (FN)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Neurofibrillary tangles are the hallmark lesions of Alzheimer and quote;s disease (AD). These tangles contain Paired Helical Filaments (PHF), which are insoluble structures composed of a highly phosphorylated form of the microtubule-associated protein tau, and associated lipid. Tau romotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.