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|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse|
|Immunogen||Synthetic peptide corresponding to a C-terminal fragment of rat neurofilament.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||3-5 ug/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:500|
|Immunohistochemistry (PFA fixed) (IHC (PFA))||Assay Dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
MA1-2011 detects the neurofilament, medium chain in human and rat samples.
MA1-2011 has been successfully used in Western blot, immunofluorescent, and immunoprecipitation procedures. By Western blot this antibody detects a ~160 kDa protein representing the neurofilament, medium chain in HEK 293 cells, rat spinal cord, and human frontal cortex. In immunofluorecence procedures MA1-2011 recognizes the neurofilament, medium chain in human neurons.
The MA1-2011 immunogen is a synthetic peptide corresponding to a C-terminal fragment of rat neurofilament.
Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (~70, 160, and 200 kDa) occur in both the central and
peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells.
MA1-2011 was used in immunocytochemistry to transform human neuronal cells by means of human adenovirus and compare these cell lines with HEK 293 cells
|Shaw G,Morse S,Ararat M,Graham FL||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (16:869)||2002|
160 kD neurofilament protein; 160 kDa neurofilament protein; NEF3; neurofilament 3; neurofilament 3, medium; neurofilament medium polypeptide; Neurofilament protein, middle polypeptide; neurofilament triplet M protein; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); NF-M; NFM; NMC
NEF3; NEFM; NF-M; NFM