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Neurogenin 3 Antibody (BS-0922R) in IHC (P)
paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval with 0.4% pepsin at 37°C for 30min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Neurogenin 3 Polyclonal Antibody, Unconjugated (bs-0922R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining... View More
Product Details
BS-0922R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human NGN3, amino acids 61-160.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Neurog 3 protein contains one basic helix-loop-helix (bHLH) domain.It is both necessary and sufficient to induce endocrine islet cell differentiation from embryonic pancreatic progenitors. Not only does itinitiates endocrine cell differentiation, italso promotes islet cell maturation and maintains islet function. It is reported that Neurogenin 3 can redirect the differentiation of bipotential secretory progenitors to endocrine rather than goblet cell fate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: atonal homolog 5; bHLHa7; Class A basic helix-loop-helix protein 7; Helix-loop-helix protein mATH-4B; mATH4B; Neurogenin 3 (Atonal protein homolog 5) (Helix-loop-helix protein mATH-4B) (MATH4B); Neurogenin-3; neurogenin3; NGN-3; Protein atonal homolog 5; transcriptional regulator, Relax
Gene Aliases: Ath4b; ATOH5; BHLHA7; Math4B; NEUROG3; NGN-3; NGN3; Relax
UniProt ID: (Human) Q9Y4Z2, (Mouse) P70661
Entrez Gene ID: (Human) 50674, (Pig) 110256612, (Rat) 60329, (Mouse) 11925
RNA polymerase II core promoter proximal region sequence-specific DNA binding
core promoter binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription coactivator activity
protein binding
chromatin DNA binding
protein dimerization activity
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
DNA binding
basic helix-loop-helix transcription factor
negative regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
nervous system development
central nervous system development
peripheral nervous system development
spinal cord development
epithelial cell differentiation
forebrain development
hindbrain development
endocrine pancreas development
positive regulation of neuron differentiation
positive regulation of transcription from RNA polymerase II promoter
regulation of dendrite morphogenesis
positive regulation of sequence-specific DNA binding transcription factor activity
transdifferentiation
regulation of transcription, DNA-templated
multicellular organism development
positive regulation of cell differentiation
transcription, DNA-templated
cell differentiation
Performance Guarantee
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