|Blocking Assay (BLOCK)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Species reactivity||Not Applicable|
|Host / Isotype||Goat / Ig Mix|
|Storage buffer||whole serum diluted in PBS, pH 7.6|
|Contains||0.05% sodium azide|
|Storage conditions||4° C|
For blocking purposes, use 5% (v/v) solution (1:20 dilution from rehydrated volume). Rehydration according to instruction yields 100% serum.
Restoration and Storage: Store product at 4°C until opened. Restore with 2.0 ml distilled water. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. Product may be stored for up to several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day. To extend the shelf-life of this product, freeze rehydrated undiluted product in small aliquots at -20°C.
Country of Origin: USA
Normal Sera are non-immune serum samples from goat, human, mouse, rabbit and other species that contain the usual complement of serum proteins, including the various classes of immunoglobulins that exist in healthy animals of the particular species. Normal serum provides a sufficient quantity of endogenous proteins to saturate and block nonspecific binding sites on fixed samples or assay substrates. When used as an antibody diluent, non-immune serum also supplies a native chemical environment for antibodies to function properly without adding foreign protein components. Normal serum is frequently used for blocking or saturating generalized binding interactions for immunodetection methods, especially those involving tissue samples such as immunohistochemistry (IHC). Normal sera are also useful as controls for testing general and specific antibody purification methods.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Whole serum
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.