|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant protein encoding the ligand binding region of human Notch 1|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-91405 detects Notch1 from mouse and human samples.
MA1-91405 has been successfully used in flow cytometry and immunohistochemistry (paraffin tissue) applications.Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at room temperature for 20 min. A suggested positive control for this product is pancreas.
The MA1-91405 immunogen is recombinant protein encoding the ligand binding region of human Notch 1.
The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine rich EGF like repeats in the extracellular region. Four notch genes have been described in mammals, which include notch 1. notch 2, notch 3, and notch 4(Int 3), which have been implicated in the differentiation of the nervous system and other structures. The EGF like proteins Delta and Serrate have been identified as ligands of notch 1. Mature notch proteins are heterodimeric receptors derived from the cleavage of notch preproteins into an extracellular subunit (NEC) containing multiple EGF like repeats and a transmembrane subunit including intracellular region (Ntm). Alteration of notch 1 signaling or expression may contribute to tumorigenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Notch1 modulates mesenchymal stem cells mediated regulatory T-cell induction.
MA1-91405 was used in blocking or activating experiment to study the mechanism by which Notch1 regulates regulatory T-cell induction by mesenchymal stem cells
|Del Papa B,Sportoletti P,Cecchini D,Rosati E,Balucani C,Baldoni S,Fettucciari K,Marconi P,Martelli MF,Falzetti F,Di Ianni M||European journal of immunology (43:182)||2013|
Blockage of Notch1 signaling modulates the T-helper (Th)1/Th2 cell balance in chronic hepatitis B patients.
MA1-91405 was used in blocking or activating experiment to investigate the influence of blockage of Notch1 signaling on T-helper cell balance in patients with chronic hepatitis B viral infection
|Pei J,Tang Z,Zang G,Yu Y||Hepatology research : the official journal of the Japan Society of Hepatology (40:799)||2010|
|Not Applicable||Not Cited||
Distinct roles of EGF repeats for the Notch signaling system.
MA1-91405 was used in blocking or activating experiment to report that Notch1 forms homodimers via its EGF motifs
|Sakamoto K,Chao WS,Katsube K,Yamaguchi A||Experimental cell research (302:281)||2005|
Distinct spatial and molecular features of notch pathway assembly in regulatory T cells.
MA1-91405 was used in flow cytometry to study the distinct molecular features of the Notch pathway in regulatory T cells
|Perumalsamy LR,Marcel N,Kulkarni S,Radtke F,Sarin A||Science signaling (5:null)||2012|
Candidate genes on chromosome 9q33-34 involved in the progression of childhood ependymomas.
MA1-91405 was used in flow cytometry to identify genetic alterations in childhood ependymomas
|Puget S,Grill J,Valent A,Bieche I,Dantas-Barbosa C,Kauffmann A,Dessen P,Lacroix L,Geoerger B,Job B,Dirven C,Varlet P,Peyre M,Dirks PB,Sainte-Rose C,Vassal G||Journal of clinical oncology : official journal of the American Society of Clinical Oncology (27:1884)||2009|
Changes in the expression of stem cell markers in oral lichen planus and hyperkeratotic lesions.
MA1-91405 was used in immunohistochemistry to investigate the expression of stem cell markers in normal and diseased buccal mucosa
|Köse O,Lalli A,Kutulola AO,Odell EW,Waseem A||Journal of oral science (49:133)||2007|
Pancreatic regeneration in chronic pancreatitis requires activation of the notch signaling pathway.
MA1-91405 was used in immunohistochemistry to study the role of Notch signaling in pancreatic regeneration in chronic pancreatitis
|Su Y,Büchler P,Gazdhar A,Giese N,Reber HA,Hines OJ,Giese T,Büchler MW,Friess H||Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract (10:1230)||2006|
Sarcomatoid carcinomas of the lung--are these histogenetically heterogeneous tumors?
MA1-91405 was used in immunohistochemistry to study the histogenetic heterogeneity of sarcomatoid carcinomas of the lung
|Blaukovitsch M,Halbwedl I,Kothmaier H,Gogg-Kammerer M,Popper HH||Virchows Archiv : an international journal of pathology (449:455)||2006|
The Notch signaling pathway is related to neurovascular progression of pancreatic cancer.
MA1-91405 was used in immunohistochemistry to study the role of the Notch pathway in pancreatic cancer angiogenesis and neuroinvasion
|Büchler P,Gazdhar A,Schubert M,Giese N,Reber HA,Hines OJ,Giese T,Ceyhan GO,Müller M,Büchler MW,Friess H||Annals of surgery (242:791)||2005|
Isolation of precursor cells (PCs) from human dental follicle of wisdom teeth.
MA1-91405 was used in immunohistochemistry to isolate and characterize precursor cells from human dental follicle of wisdom teeth
|Morsczeck C,Götz W,Schierholz J,Zeilhofer F,Kühn U,Möhl C,Sippel C,Hoffmann KH||Matrix biology : journal of the International Society for Matrix Biology (24:155)||2005|
Evidence for a role for notch signaling in the cytokine-dependent survival of activated T cells.
MA1-91405 was used in western blot to study the potential role of Notch1 signaling in cytokine-dependent survival of activated T cells
|Bheeshmachar G,Purushotaman D,Sade H,Gunasekharan V,Rangarajan A,Sarin A||Journal of immunology (Baltimore, Md. : 1950) (177:5041)||2006|
NB-3/Notch1 pathway via Deltex1 promotes neural progenitor cell differentiation into oligodendrocytes.
MA1-91405 was used in western blot to study the involvement of NB-3/Notch1 pathway in the development of oligodendrocytes
|Cui XY,Hu QD,Tekaya M,Shimoda Y,Ang BT,Nie DY,Sun L,Hu WP,Karsak M,Duka T,Takeda Y,Ou LY,Dawe GS,Yu FG,Ahmed S,Jin LH,Schachner M,Watanabe K,Arsenijevic Y,Xiao ZC||The Journal of biological chemistry (279:25858)||2004|