Western blot analysis of Notch1 in HEK293 cells transfected with full length Notch 1 A) untreated and B) treated with 5mM EDTA for 15 minutes, using a Notch1/Notch1 cleaved polyclonal antibody (Product # PA5-23181) at 3ug/ml. The EDTA treatment increases the detected level of Notch1-cv.
|Tested species reactivity||Bovine, Dog, Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (1755 VLLSRKRRRQHGQC 1767) of Notch1/Notch1 cleaved protein.|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||3-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The Notch protein family contains a group of highly conserved single-pass, ligand-activated transmembrane receptors involved in cell fate selection and maintenance of progenitors during development. These proteins also function as transcription factors. Notch proteins mainly consist of an extra cellular domain containing tandem EGF-repeats and three lin-12/Notch repeats, a transmembrane domain and an intracellular domain containing six cdc1/SW16/ankyrin repeats, RAM motif, nuclear localization signals and a C-terminal OPA/PEST region. NOTCH1, a mammalian homolog of Drosophila Notch, is an important member of this family. Physiological functions influenced by NOTCH proteins include maintenance of hematopoietic stem cells, T-cell development, neuronal function and development, angiogenesis, arterial and ventricular development and differentiation, cardiac valve homeostasis, cell lineage specification of endocrine and exocrine pancreas, development of actin cytoskeleton and bone regeneration. Abnormal Notch signaling has been associated with several diseases including cancer, leukemia, melanoma, T-ALL, CADASIL, Multiple Sclerosis, Tetralogy of Fallot and Alagille Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.