|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Full length human protein.|
|Storage buffer||PBS, pH 7.2, with 1% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1250-1:2500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
MA1-26189 detects ORC1 from human samples.
MA1-26189 has been successfully used in Western blot procedures.
The MA1-26189 immunogen is full length human protein.
The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Localization of ORC1 during the cell cycle in human leukemia cells.
MA1-26189 was used in immunocytochemistry to study the localization of ORC1 in human leukemia cells during the cell cycle
|Coffman FD,Reyes ML,Brown M,Lambert WC,Cohen S||Analytical cellular pathology (Amsterdam) (34:355)||2011|