Immunofluorescent analysis of Occludin was performed on 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ABfinity™ Occludin recombinant rabbit monoclonal antibody (Product # 701161) at a dilution of 1:500 in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour® 488 goat anti-rabbit IgG secondary antibody (Product # A11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c is a merged image and showing cell junction localization and panel d is a control without primary antibody. The images were captured using a Nikon microscope at 20X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Pig, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 387–403 of human occludin|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with equine, mouse, rabbit and rat based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Tight junction group of proteins are a cell-to-cell adhesion structure in epithelial cells that constitute the epithelial junctional complex with adherens junctions and desmosomes. Tight junction strands are mainly composed of claudins, occludin, and JAM. Occludin is a 65 kDa protein and is considered to be vital in the assembly and maintenance of tight junctions. At the tight junction, occludin associates with membrane peripheral protein ZO-1. The expression of occludin varies greatly on the type of tissue. It is continuously and highly expressed in brain whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Early Activation of MAPK p44/42 Is Partially Involved in DON-Induced Disruption of the Intestinal Barrier Function and Tight Junction Network.
701161 was used in western blot assess the effects of deoxynivalenol and its derivatives on intestinal tight junction proteins
|Springler A,Hessenberger S,Schatzmayr G,Mayer E||Toxins (8:null)||2016|
Kiwifruit cysteine protease actinidin compromises the intestinal barrier by disrupting tight junctions.
701161 was used in western blot to assess the effects of actinidin on intestinal epithelium tight junctions
|Grozdanovic MM,¿avi¿ M,Ne¿i¿ A,Andjelkovi¿ U,Akbari P,Smit JJ,Gavrovi¿-Jankulovi¿ M||Biochimica et biophysica acta (1860:516)||2016|
|Human||1:100||Claudin 1 expression characterizes human uterine cervical reserve cells.||Zinner B,Gyöngyösi B,Babarczi E,Kiss A,Sobel G||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (61:880)||2013|
|Human||1:200||Label-free recognition of drug resistance via impedimetric screening of breast cancer cells.||Eker B,Meissner R,Bertsch A,Mehta K,Renaud P||PloS one (8:null)||2013|
|Human||1:200||Distinguishing drug-induced minor morphological changes from major cellular damage via label-free impedimetric toxicity screening.||Meissner R,Eker B,Kasi H,Bertsch A,Renaud P||Lab on a chip (11:2352)||2011|