|Western Blot (WB)||6 ug/ml|
|Tested Species reactivity||Bovine, Human, Mouse|
|Host / Isotype||Mouse / Ig Mix|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage conditions||4° C, do not freeze|
This Anti-OxPhos Complex Kit is a cocktail of monoclonal antibodies specific to human, bovine, mouse OxPhos Complex This antibody cocktail is validated for use in Western blotting.
C-I-39 (NDUFA9) (clone) 20C11B11B11 - Bovine Heart Complex I
(Fp) (clone) 2E3GC12FB2AE2 -Bovine Heart Complex II
C-III-Core 2 (clone) 13G12AF12BB11 - Bovine Heart Comp. II, III
C-IV-subunit IV (clone) 20E8C12 - Bovine Comp. IV, holoenz.
C-V-alpha (clone) 15H4C4 - Bovine Complex V
Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress. Blue Native polyacrylamide gel electrophoresis (BN-PAGE) is a simple and effective way to subfractionate mitochondrial proteins as intact complexes on a single gel (in one dimension). It can be used to detect altered assembly of these complexes arising from mutations in subunits, mutations in assembly factors, or mtDNA depletion. This type of analysis has been performed with biopsy samples, platelets and fibroblast cells from patients with suspected mitochondrial diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Oxidative Phosphorylation; OxPhos Complex
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.