|Tested species reactivity||Mouse|
|Published species reactivity||Tag, Rat, Bovine, Sheep, Fish, Human, Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2a|
|Immunogen||This antibody reacts with pro and active MMP-2 protein.|
|Purification||Ammonium sulfate precipitation, Ion-exchange chromatography|
|Storage buffer||PBS with 1% BSA|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Inhibition Assays (IA)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 2 publications below|
|Immunohistochemistry (IHC)||See 4 publications below|
|Immunofluorescence (IF)||See 4 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 2 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|In vitro Assay (IV)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 4 publications below|
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congential hypotrichosis with juvenile macular dystrophy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The epidermal polarity protein Par3 is a non-cell autonomous suppressor of malignant melanoma.
13-2000Z was used in immunohistochemistry - paraffin section and western blot to describe how epithelial polarity proteins suppress malignant outgrowth
|Mescher M,Jeong P,Knapp SK,Rübsam M,Saynisch M,Kranen M,Landsberg J,Schlaak M,Mauch C,Tüting T,Niessen CM,Iden S||The Journal of experimental medicine (214:339)||2017|
BMI1 represses Ink4a/Arf and Hox genes to regulate stem cells in the rodent incisor.
13-2000Z was used in immunohistochemistry - paraffin section to study BMI-mediated repression of Hox genes in stem cells.
|Biehs B,Hu JK,Strauli NB,Sangiorgi E,Jung H,Heber RP,Ho S,Goodwin AF,Dasen JS,Capecchi MR,Klein OD||Nature cell biology (15:846)||2013|
Retinal pigment epithelial integrity is compromised in the developing albino mouse retina.
13-2000Z was used in immunohistochemistry to utilize the developing albino mouse retina to study compromised retinal pigment epithelial integrity
|Iwai-Takekoshi L,Ramos A,Schaler A,Weinreb S,Blazeski R,Mason C||The Journal of comparative neurology (524:3696)||2016|
Cadherin expression in the developing mouse olfactory system.
13-2000Z was used in immunohistochemistry to report the localization of classical cadherins in the developing olfactory system.
|Akins MR,Benson DL,Greer CA||The Journal of comparative neurology (501:483)||2007|
|Mouse||Not Cited||Links between signal transduction, transcription and adhesion in epithelial bud development.||Jamora C,DasGupta R,Kocieniewski P,Fuchs E||Nature (422:317)||2003|
|The cadherins: cell-cell adhesion molecules controlling animal morphogenesis.||Takeichi M||Development (Cambridge, England) (102:639)||1988|
|Mouse||1:200||Keratin 79 identifies a novel population of migratory epithelial cells that initiates hair canal morphogenesis and regeneration.||Veniaminova NA,Vagnozzi AN,Kopinke D,Do TT,Murtaugh LC,Maillard I,Dlugosz AA,Reiter JF,Wong SY||Development (Cambridge, England) (140:4870)||2013|
|WT1-interacting protein and ZO-1 translocate into podocyte nuclei after puromycin aminonucleoside treatment.||Rico M,Mukherjee A,Konieczkowski M,Bruggeman LA,Miller RT,Khan S,Schelling JR,Sedor JR||American journal of physiology. Renal physiology (289:F431)||2005|
||Conditional targeting of E-cadherin in skin: insights into hyperproliferative and degenerative responses.||Tinkle CL,Lechler T,Pasolli HA,Fuchs E||Proceedings of the National Academy of Sciences of the United States of America (101:552)||2004|
||Neph1 and nephrin interaction in the slit diaphragm is an important determinant of glomerular permeability.||Liu G,Kaw B,Kurfis J,Rahmanuddin S,Kanwar YS,Chugh SS||The Journal of clinical investigation (112:209)||2003|
|Not Applicable||10 µg/ml||
Abnormal placental development and early embryonic lethality in EpCAM-null mice.
13-2000Z was used in immunohistochemistry - frozen section to review data regarding EpCAM
|Nagao K,Zhu J,Heneghan MB,Hanson JC,Morasso MI,Tessarollo L,Mackem S,Udey MC||PloS one (4:null)||2009|
P-cadherin is a p63 target gene with a crucial role in the developing human limb bud and hair follicle.
13-2000Z was used in immunohistochemistry - frozen section to investigate if p63 directly interacts with two distinct regions of the P-cadherin gene CDH3 promoter
|Shimomura Y,Wajid M,Shapiro L,Christiano AM||Development (Cambridge, England) (135:743)||2008|
|Not Applicable||Not Cited||
Tissue surface tensions guide in vitro self-assembly of rodent pancreatic islet cells.
13-2000Z was used in flow cytometry to investigate how differential adhesion alters the organization of immortalized rodent pancreatic islet cells
|Jia D,Dajusta D,Foty RA||Developmental dynamics : an official publication of the American Association of Anatomists (236:2039)||2007|
|Mouse||Not Cited||Conditional targeting of E-cadherin in skin: insights into hyperproliferative and degenerative responses.||Tinkle CL,Lechler T,Pasolli HA,Fuchs E||Proceedings of the National Academy of Sciences of the United States of America (101:552)||2004|
|Rat||Not Cited||Neph1 and nephrin interaction in the slit diaphragm is an important determinant of glomerular permeability.||Liu G,Kaw B,Kurfis J,Rahmanuddin S,Kanwar YS,Chugh SS||The Journal of clinical investigation (112:209)||2003|
Cadherin-mediated cell adhesion and tissue segregation: qualitative and quantitative determinants.
13-2000Z was used in flow cytometry to examine cadherin-subtype-specific binding specificities.
|Duguay D,Foty RA,Steinberg MS||Developmental biology (253:309)||2003|
Genetic dissection of cadherin function during nephrogenesis.
13-2000Z was used in immunohistochemistry to determine if R-cadherin is required for kidney ontogeny.
|Dahl U,Sjödin A,Larue L,Radice GL,Cajander S,Takeichi M,Kemler R,Semb H||Molecular and cellular biology (22:1474)||2002|
Ectopic expression of PA2.26 antigen in epidermal keratinocytes leads to destabilization of adherens junctions and malignant progression.
13-2000Z was used in western blot to report that expression of the PA2.26 antigen by premalignant keratinocytes induces a fully transformed and metastatic phenotype.
|Scholl FG,Gamallo C,Quintanilla M||Laboratory investigation; a journal of technical methods and pathology (80:1749)||2000|
Actin 'purse string' filaments are anchored by E-cadherin-mediated adherens junctions at the leading edge of the epithelial wound, providing coordinated cell movement.
13-2000Z was used in immunohistochemistry to elucidate the role of the actin filament cable in wound healing.
|Danjo Y,Gipson IK||Journal of cell science (111 ( Pt 22):3323)||1998|