For immunohistochemistry (paraffin) applications, it is recommended to microwave samples prior to staining. For flow cytometry, incubate overnight at 4°C.
Reconstitute with 50-100 µL of distilled water, and dilute further in 0.1M PBS with 1% BSA and 0.1% sodium azide. For long-term storage, aliquot and store at -20°C.
VACHt is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.
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Protein Aliases: solute carrier family 18 (vesicular acetylcholine), member 3; solute carrier family 18 (vesicular monoamine) member 3; Solute carrier family 18 member 3; solute carrier family 18, member 3; VAChT; VAT; Vesicular acetylcholine transporter
Gene Aliases: rVAT; Slc18a3; VAChT; VAT
UniProt ID: (Rat) Q62666
Molecular Function: amino acid transporter