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FIGURE: 1 / 1
eIF1 Antibody (PA5-88038) in WB
Western blot analysis of eIF1 in extracts of various cell lines. Samples were incubated with eIF1 Polyclonal antibody (Product # PA5-88038) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-88038
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-113 of human EIF1 (NP_0057921)
Conjugate
Form
Liquid
Concentration
2.08 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804608
Product Specific Information
Immunogen sequence: MSAIQNLHSF DPFADASKGD DLLPAGTEDY IHIRIQQRNG RKTLTTVQGI ADDYDKKKLV KAFKKKFACN GTVIEHPEYG EVIQLQGDQR KNICQFLVEI GLAKDDQLKV HGF; Positive Samples: A-549, Mouse liver
Target Information
Necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A121; eIF1; eIF13; Eukaryotic translation initiation factor 1; Protein translation factor SUI1 homolog; Sui1iso1; suppressor of initiator codon mutations, related sequence 1; suppressor of initiator codon mutations-Yeast homolog related sequence 1
Gene Aliases: A121; EIF-1; EIF1; EIF1A; ISO1; SUI1; Sui1-rs1
UniProt ID: (Human) P41567, (Mouse) P48024
Entrez Gene ID: (Human) 10209, (Mouse) 20918
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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