|Tested species reactivity||Chicken, Human, Rabbit, Xenopus|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant PABP (Human) expressed from its 1.85 kbp cDNA, NcoI to SspI.|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunofluorescence (IF)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-34079 detects PABP from human, chicken, rabbit and Xenopus laevis samples. Does not react wtih Drosophilia melanogaster or mouse samples.
MA1-34079 has been successfully used in immunocytochemistry, immunofluorescence, immunoprecipitation, Western blot and ELISA applications.
The MA1-34079 immunogen is Recombinant PABP (Human) expressed from its 1.85 kbp cDNA, NcoI to SspI.
Store at 4°C. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. In humans, the PABPs comprise a small nuclear isoform and a conserved gene family that displays at least 3 functional proteins: PABP1 (PABPC1), inducible PABP (iPABP, or PABPC4; MIM 603407), and PABP3 (PABPC3; MIM 604680). In addition, there are at least 4 pseudogenes, PABPCP1 to PABPCP4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.