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PAK3 Antibody (MA5-38059) in IHC (P)
Immunohistochemistry analysis of PAK3 in paraffin-embedded mouse brain. Samples were incubated with PAK3 Monoclonal antibody (Product # MA5-38059) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Product Details
MA5-38059
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC1955
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PAK3 (O75914)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.58 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897977
Product Specific Information
Positive Samples: SH-SY5Y, Mouse brain, Rat brain
Immunogen sequence: MSDGLDNEEK PPAPPLRMNS NNRDSSALNH SSKPLPMAPE EKNKKARLRS IFPGGGDKTN KKKEKERPEI SLPSDFEHTI HVGFDAVTGE FTPDLYGSQM
Target Information
PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gammaPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gammaPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2.7.11.1; Beta-PAK; CDC42/RAC effector kinase PAK-B; CDKN1A; hPAK3; p21 (CDKN1A)-activated kinase 3; p21-activated kinase 3; p21/Cdc42/Rac1-activated kinase 3 (yeast Ste20-related); p65-PAK; PAK beta; PAK-3; Serine/threonine-protein kinase PAK 3
Gene Aliases: beta-PAK; PAK-3; Pak3; Pak65alpha; Pak65beta; Pakb; Stk4
UniProt ID: (Mouse) Q61036, (Rat) Q62829
Entrez Gene ID: (Mouse) 18481, (Rat) 29433
nucleotide binding
catalytic activity
protein kinase activity
protein serine/threonine kinase activity
MAP kinase kinase activity
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Rho GTPase binding
SH3 domain binding
metal ion binding
Rac GTPase binding
GTPase binding
receptor signaling protein serine/threonine kinase activity
MAPK cascade
activation of MAPK activity
protein phosphorylation
Rho protein signal transduction
multicellular organism development
regulation of mitotic cell cycle
axonogenesis
metabolic process
regulation of neuron projection development
phosphorylation
dendrite development
cell migration
signal transduction by protein phosphorylation
actin cytoskeleton organization
regulation of actin filament polymerization
stress-activated protein kinase signaling cascade
activation of protein kinase activity
regulation of apoptotic process
regulation of MAPK cascade
positive regulation of neuron apoptotic process
dendritic spine development
positive regulation of dendritic spine morphogenesis
cellular response to organic cyclic compound
positive regulation of DNA biosynthetic process
mitotic cell cycle
intracellular signal transduction
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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