Immunohistochemistry was performed on formalin-fixed paraffin-embedded canine lymph node tissue sections. Antigen retrieval was performed by heating tissues in 10mM sodium citrate buffer, pH 9.0 for 20 minutes. Tissues were blocked in 10% normal goat serum containing 2X casein for 30 minutes at room temperature and probed with a PAX5 monoclonal antibody, clone SP34 (Product # MA1-39420) for 1.5 hours at 37°C. Detection was performed using a biotinylated goat anti-rabbit IgG secondary antibody description at a dilution of 1:200 for 30 minutes at room temperature followed by streptavidin-HRP for 20 minutes at room temperature and a chromogenic substrate. Tissues were visualized by light microscopy. Data courtesy of the Innovators Program.
|Tested species reactivity||Dog, Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide from C-terminus of human Pax-5 protein|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 2 publications below|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.
This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Increased utilization, verification, and clinical implications of immunocytochemistry: Experience in a northern New England hospital.
MA1-39420 was used in immunohistochemistry to study a northern New England hospital and increased utilization, verification, and clinical implications of immunocytochemistry
|Sauter JL,Ambaye AB,Mount SL||Diagnostic cytopathology (43:688)||2015|
Composite lymphoma in the anterior mediastinum: a case report and review of the literature.
MA1-39420 was used in immunohistochemistry to present a case report of an unusual composite lymphoma in the mediastinum
|Yu G,Kong L,Qu G,Zhang Q,Wang W,Jiang L||Diagnostic pathology (6:null)||2011|