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|Tested species reactivity||Bovine, Human, Mouse, Rat|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human recombinant OSCP and PDH E1-alpha|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ELISA (ELISA)||8 µg/ml|
|Flow Cytometry (Flow)||1 µg/ml|
|Immunocytochemistry (ICC)||5 µg/ml|
|Immunofluorescence (IF)||5 µg/ml|
|Western Blot (WB)||1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
For positive control, use isolated mitochondria from Human, Bovine, Rat and Mouse hearts, HepG2 lysate; cultured, normal Human embryonic lung fibroblasts (strain MRC5); HL60 cells.
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mutant Kras copy number defines metabolic reprogramming and therapeutic susceptibilities.
459400 was used in western blot to elucidate the mechanisms by which KRAS mutations contribute to lung cancer.
|Kerr EM,Gaude E,Turrell FK,Frezza C,Martins CP||Nature (531:110)||2016|
Ras-mediated modulation of pyruvate dehydrogenase activity regulates mitochondrial reserve capacity and contributes to glioblastoma tumorigenesis.
459400 was used in western blot to discuss the metabolic programs that drive tumorigenesis.
|Prabhu A,Sarcar B,Miller CR,Kim SH,Nakano I,Forsyth P,Chinnaiyan P||Neuro-oncology (17:1220)||2015|
E1-alpha polypeptide 1; mitochondrial; PDHA; PDHCE1A; PDHE1-A type I; PHE1A; pyruvate dehydrogenase complex; pyruvate dehydrogenase complex, E1-alpha polypeptide 1; pyruvate dehydrogenase E1 alpha 1; pyruvate dehydrogenase E1 component alpha subunit; pyruvate dehydrogenase E1 component subunit alpha; pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; pyruvate dehydrogenase E1 subunit; pyruvate dehydrogenase E1alpha subunit; somatic form
BOS_26312; PDHA; Pdha-1; PDHA1; PDHAD; PDHCE1A; PHE1A