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Western blot analysis of PEX19 using A) 30 µg Jurkat whole cell lysate (B) 30 µg Raji whole cell lysate (C) 30 µg K562 whole cell lysate (D) 30 µg THP-1 whole cell lysate and E) 30 µg NCI-H929 whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a PEX19 monoclonal antibody (Product # MA5-17281) at a dilution of 1:1000.
|Tested species reactivity||Human, Rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant fragment contains a sequence corresponding to a region within amino acids 15 and 299 of PEX19|
|Storage buffer||PBS, pH 7|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17281 targets PEX19 in WB applications and shows reactivity with Human and Rat samples.
The MA5-17281 immunogen is recombinant fragment contains a sequence corresponding to a region within amino acids 15 and 299 of PEX19.
Necessary for early peroxisomal biogenesis. Acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Binds and stabilizes newly synthesized PMPs in the cytoplasm by interacting with their hydrophobic membrane-spanning domains, and targets them to the peroxisome membrane by binding to the integral membrane protein PEX3. Excludes CDKN2A from the nucleus and prevents its interaction with MDM2, which results in active degradation of TP53.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
33 kDa housekeeping protein; 33kD; D1S2223E; HK33; housekeeping gene; housekeeping gene, 33kD; peroxin-19; peroxisomal biogenesis factor 19; peroxisomal farnesylated protein; peroxisome biogenesis factor 19; PMP1; PMPI; PXF; PXMP1
D1S2223E; HK33; OK/SW-cl.22; PBD12A; PEX19; PMP1; PMPI; PXF; PXMP1