Product References
Aft1 Nuclear Localization and Transcriptional Response to Iron Starvation Rely upon TORC2/Ypk1 Signaling and Sphingolipid Biosynthesis.
International journal of molecular sciences
Montellà-Manuel S,Pujol-Carrion N,de la Torre-Ruiz MA
459250 was used in Western Blotting to reveal a novel role for sphingolipids, controlled by TORC2-Ypk1, for proper localization and activity of Aft1 in response to iron scarcity.
Thu Jan 26 00:00:00 EST 2023
Rio1 downregulates centromeric RNA levels to promote the timely assembly of structurally fit kinetochores.
Nature communications
Smurova K,Damizia M,Irene C,Stancari S,Berto G,Perticari G,Iacovella MG,D'Ambrosio I,Giubettini M,Philippe R,Baggio C,Callegaro E,Casagranda A,Corsini A,Polese VG,Ricci A,Dassi E,De Wulf P
459250 was used in Western Blotting to suggest that CEN regulation by Rio1/RioK1 is evolutionary conserved.
Thu Jun 01 00:00:00 EDT 2023
Genetic requirements for repair of lesions caused by single genomic ribonucleotides in S phase.
Nature communications
Schindler N,Tonn M,Kellner V,Fung JJ,Lockhart A,Vydzhak O,Juretschke T,Möckel S,Beli P,Khmelinskii A,Luke B
459250 was used in Western Blotting to study the mechanisms involving nick lesion repair (NLR), and conclude that the NLR genetic network may have important implications in the context of human pathologies.
Fri Mar 03 00:00:00 EST 2023
Genipin prevents alpha-synuclein aggregation and toxicity by affecting endocytosis, metabolism and lipid storage.
Nature communications
Rosado-Ramos R,Poças GM,Marques D,Foito A,M Sevillano D,Lopes-da-Silva M,Gonçalves LG,Menezes R,Ottens M,Stewart D,Ibáñez de Opakua A,Zweckstetter M,Seabra MC,Mendes CS,Outeiro TF,Domingos PM,Santos CN
459250 was used in Western Blotting to identify genipin, an iridoid, which relieves αSyn toxicity and aggregation.
Thu Apr 06 00:00:00 EDT 2023
Hsp90 mutants with distinct defects provide novel insights into cochaperone regulation of the folding cycle.
PLoS genetics
Mercier R,Yama D,LaPointe P,Johnson JL
459250 was used in Western Blotting to indicate that the timing of transition into and out of the closed conformation is tightly regulated by cochaperones.
Mon May 01 00:00:00 EDT 2023
Identification of 14-3-3 proteins, Polo kinase, and RNA-binding protein Pes4 as key regulators of meiotic commitment in budding yeast.
Current biology : CB
Gavade JN,Puccia CM,Herod SG,Trinidad JC,Berchowitz LE,Lacefield S
459250 was used in Western Blot to demonstrate that meiotic commitment is actively maintained throughout meiosis, with the 14-3-3 proteins and Polo kinase serving as key regulators of this developmental program.
Mon Apr 11 00:00:00 EDT 2022
Whi3 mnemon association with endoplasmic reticulum membranes confines the memory of deceptive courtship to the yeast mother cell.
Current biology : CB
Lau Y,Oamen HP,Grogg M,Parfenova I,Saarikangas J,Hannay R,Nichols RA,Hilvert D,Barral Y,Caudron F
459250 was used in Western Blotting to suggest that confined self-templating super-assembly is a powerful mechanism for the long-term encoding of information in a spatially defined manner.
Mon Mar 14 00:00:00 EDT 2022
Duplicated ribosomal protein paralogs promote alternative translation and drug resistance.
Nature communications
Malik Ghulam M,Catala M,Reulet G,Scott MS,Abou Elela S
459250 was used in Western Blotting to demonstrate that gene duplication may serve as a stress-adaptation mechanism modulating the global proteome through the differential expression of ribosomal protein paralogs.
Tue Aug 23 00:00:00 EDT 2022
Both human and soya bean ferritins highly improve the accumulation of bioavailable iron and contribute to extend the chronological life in budding yeast.
Microbial biotechnology
Pujol-Carrion N,Gonzalez-Alfonso A,Puig S,de la Torre-Ruiz MA
459250 was used in Western Blotting to demonstrate that the four human and soya bean ferritin chains are successfully expressed in our model system.
Sun May 01 00:00:00 EDT 2022
Switching a conflicted bacterial DTD-tRNA code is essential for the emergence of mitochondria.
Science advances
Gogoi J,Bhatnagar A,Ann KJ,Pottabathini S,Singh R,Mazeed M,Kuncha SK,Kruparani SP,Sankaranarayanan R
459250 was used in Western Blotting to show that unlike bacterial tRNAGly, eukaryotes harbor adenine at the N73rd position of cytoplasmic and mitochondrial tRNAGly.
Fri Jan 14 00:00:00 EST 2022
Protein fluorescent labeling in live yeast cells using scFv-based probes.
Cell reports methods
Tsirkas I,Zur T,Dovrat D,Cohen A,Ravkaie L,Aharoni A
459250 was used in Western Blotting to develop a single-chain fragment variable (scFv)-FP approach to efficiently label Saccharomyces cerevisiae proteins that are tagged with repeats of hemagglutinin (HA)-tag sequences.
Mon Dec 19 00:00:00 EST 2022
Mre11-Rad50 oligomerization promotes DNA double-strand break repair.
Nature communications
Kissling VM,Reginato G,Bianco E,Kasaciunaite K,Tilma J,Cereghetti G,Schindler N,Lee SS,Guérois R,Luke B,Seidel R,Cejka P,Peter M
459250 was used in Western Blot to demonstrate that Mre11-Rad50-Xrs2 oligomerization facilitates foci formation, DNA damage signaling, repair, and telomere maintenance in vivo.
Mon May 02 00:00:00 EDT 2022
Molecular determinants and modifiers of Matrin-3 toxicity, condensate dynamics, and droplet morphology.
iScience
Sprunger ML,Lee K,Sohn BS,Jackrel ME
459250 was used in Western Blot to report the development of a yeast model of MATR3 proteotoxicity and aggregation.
Fri Mar 18 00:00:00 EDT 2022
Lsm7 phase-separated condensates trigger stress granule formation.
Nature communications
Lindström M,Chen L,Jiang S,Zhang D,Gao Y,Zheng J,Hao X,Yang X,Kabbinale A,Thoma J,Metzger LC,Zhang DY,Zhu X,Liu H,Gustafsson CM,Burmann BM,Winderickx J,Sunnerhagen P,Liu B
459250 was used in Western Blotting to find that deletion of one of the Pab1 co-localizing proteins, Lsm7, leads to a significant decrease in SG formation.
Tue Jun 28 00:00:00 EDT 2022
Identification of an E3 ligase that targets the catalytic subunit of RNA Polymerase I upon transcription stress.
The Journal of biological chemistry
Pitts S,Liu H,Ibrahim A,Garg A,Felgueira CM,Begum A,Fan W,Teh S,Low JY,Ford B,Schneider DA,Hay R,Laiho M
459250 was used in Western Blotting to reveal the regulated turnover of Pol I, showing that the stability of the catalytic subunit is controlled by the F-box protein FBXL14 in response to transcription stress.
Thu Dec 01 00:00:00 EST 2022
The cap-proximal RNA secondary structure inhibits preinitiation complex formation on HAC1 mRNA.
The Journal of biological chemistry
Uppala JK,Sathe L,Chakraborty A,Bhattacharjee S,Pulvino AT,Dey M
459250 was used in Western Blot to study the translational derepression mechanism found in the budding of Saccharomyces cerevisiae.
Tue Mar 01 00:00:00 EST 2022
Smc3 acetylation, Pds5 and Scc2 control the translocase activity that establishes cohesin-dependent chromatin loops.
Nature structural & molecular biology
Bastié N,Chapard C,Dauban L,Gadal O,Beckouët F,Koszul R
459250 was used in Western Blotting to investigate and provide a comprehensive overview of the roles of various cohesin regulators in tuning chromatin loop expansion in budding yeast Saccharomyces cerevisiae.
Wed Jun 01 00:00:00 EDT 2022
The role of bivalent ions in the regulation of D-loop extension mediated by DMC1 during meiotic recombination.
iScience
Altmannova V,Spirek M,Orlic L,Jēkabsons A,Clarence T,Henggeler A,Mlcouskova J,Chaleil RAG,Matos J,Krejci L
459250 was used in Western Blotting to show that whereas calcium ions in the presence of ATP cause a conformational change within DMC1, stimulating its DNA binding and D-loop formation, they inhibit the extension of the invading strand within the D-loop.
Fri Nov 18 00:00:00 EST 2022
Assembly checkpoint of the proteasome regulatory particle is activated by coordinated actions of proteasomal ATPase chaperones.
Cell reports
Nahar A,Sokolova V,Sekaran S,Orth JD,Park S
459250 was used in Western Blotting to show that chaperones may sense ATP binding and hydrolysis as a readout for the quality of the RP complex to generate a functional proteasome holoenzyme and provide a basis to potentially exploit the assembly checkpoints in situations with known deregulation of proteasomal ATPase chaperones.
Tue Jun 07 00:00:00 EDT 2022
Cdc48Ufd1/Npl4 segregase removes mislocalized centromeric histone H3 variant CENP-A from non-centromeric chromatin.
Nucleic acids research
Ohkuni K,Gliford L,Au WC,Suva E,Kaiser P,Basrai MA
459250 was used in Immunoprecipitation to show that given the conservation of Cdc48Ufd1/Npl4 in humans, it is likely that defects in such pathways may contribute to CIN in human cancers.
Fri Apr 08 00:00:00 EDT 2022
Proteaphagy is specifically regulated and requires factors dispensable for general autophagy.
The Journal of biological chemistry
Waite KA,Burris A,Vontz G,Lang A,Roelofs J
459250 was used in Western Blot to study the molecular regulation of proteaphagy in response to small molecule and genetic perturbations.
Sat Jan 01 00:00:00 EST 2022
The Zip4 protein directly couples meiotic crossover formation to synaptonemal complex assembly.
Genes & development
Pyatnitskaya A,Andreani J,Guérois R,De Muyt A,Borde V
459250 was used in Western Blotting to show that Ecm11, a SC central element protein, localizes on both DSB sites and sites that attach chromatin loops to the chromosome axis, which are the starting points of SC formation, in a way that strictly requires the ZMM protein Zip4.
Sat Jan 01 00:00:00 EST 2022
Sequestration of TDP-43216-414 Aggregates by Cytoplasmic Expression of the proSAAS Chaperone.
ACS chemical neuroscience
Peinado JR,Chaplot K,Jarvela TS,Barbieri EM,Shorter J,Lindberg I
459250 was used in Western Blotting to that proSAAS can act as a functional holdase for TDP-43 via this phase-separation property, representing a cytoprotectant whose unusual biochemical properties can potentially be exploited in the design of therapeutic molecules.
Wed Jun 01 00:00:00 EDT 2022
A yeast suppressor screen links Coa4 to the mitochondrial copper delivery pathway for cytochrome c oxidase.
Genetics
Swaminathan AB,Soma S,Vicary AC,Zulkifli M,Kaur H,Gohil VM
459250 was used in Western Blotting to provide genetic evidences for the role of Coa4 in the copper delivery pathway to the CuB site of cytochrome c oxidase.
Sat Jul 30 00:00:00 EDT 2022
Distinguishing between recruitment and spread of silent chromatin structures in Saccharomyces cerevisiae.
eLife
Brothers M,Rine J
459250 was used in Western Blot to track the positions first methylated after induction and found that repression of genes at HML and HMR began before Sir3 occupied the entire locus.
Mon Jan 24 00:00:00 EST 2022
Rif1 regulates telomere length through conserved HEAT repeats.
Nucleic acids research
Shubin CB,Mayangsari R,Swett AD,Greider CW
459250 was used in Western Blot to suggest that conserved amino acids in the region from 436 to 577 play a functional role in telomere length regulation, which is separate from their proposed DNA binding function.
Mon Apr 19 00:00:00 EDT 2021
Meiotic Cells Counteract Programmed Retrotransposon Activation via RNA-Binding Translational Repressor Assemblies.
Developmental cell
Laureau R,Dyatel A,Dursuk G,Brown S,Adeoye H,Yue JX,De Chiara M,Harris A,Ünal E,Liti G,Adams IR,Berchowitz LE
459250 was used in Western Blotting to demonstrate that the transcriptional activation of Ty3/Gypsy retrotransposons and host defence are controlled by master meiotic regulators.
Mon Jan 11 00:00:00 EST 2021
Vps34 and TOR Kinases Coordinate HAC1 mRNA Translation in the Presence or Absence of Ire1-Dependent Splicing.
Molecular and cellular biology
Uppala JK,Bhattacharjee S,Dey M
459250 was used in Western Blot to suggest that the cytosolic splicing and the translational derepression of HAC1 mRNA are coordinated by unique and parallel networks of signaling pathways.
Wed Jun 23 00:00:00 EDT 2021
TOM20-mediated transfer of Bcl2 from ER to MAM and mitochondria upon induction of apoptosis.
Cell death & disease
Lalier L,Mignard V,Joalland MP,Lanoé D,Cartron PF,Manon S,Vallette FM
459250 was used in Western Blotting to investigate the mechanisms that link the ER-mitochondria contacts, called mitochondria-associated membranes, and apoptosis by examining the subcellular localization of Bcl2 family members.
Mon Feb 15 00:00:00 EST 2021
Genetic Analysis of Sirtuin Deacetylases in Hyphal Growth of Candida albicans.
mSphere
Zhao G,Rusche LN
459250 was used in Western Blot to study the roles of three sirtuin deacetylases-Sir2, Hst1, and Hst2-in the hyphal growth of C. albicans.
Wed May 05 00:00:00 EDT 2021
Epigenetics Identifier screens reveal regulators of chromatin acylation and limited specificity of acylation antibodies.
Scientific reports
Kollenstart L,van der Horst SC,Vreeken K,Janssen GMC,Martino F,Vlaming H,van Veelen PA,van Leeuwen F,van Attikum H
459250 was used in Western Blot to reveal limits of using pan-K-acyl antibodies in epigenetics research.
Thu Jun 17 00:00:00 EDT 2021
Epigenetics Identifier screens reveal regulators of chromatin acylation and limited specificity of acylation antibodies.
Scientific reports
Kollenstart L,van der Horst SC,Vreeken K,Janssen GMC,Martino F,Vlaming H,van Veelen PA,van Leeuwen F,van Attikum H
459250 was used in Western Blot to reveal limits of using pan-K-acyl antibodies in epigenetics research.
Thu Jun 17 00:00:00 EDT 2021
Actin Cytoskeleton Regulation by the Yeast NADPH Oxidase Yno1p Impacts Processes Controlled by MAPK Pathways.
Antioxidants (Basel, Switzerland)
Weber M,Basu S,González B,Greslehner GP,Singer S,Haskova D,Hasek J,Breitenbach M,W Gourlay C,Cullen PJ,Rinnerthaler M
459250 was used in Western Blotting to show Yno1p-derived H2O2 regulates outputs controlled by three MAP kinase pathways.
Mon Feb 22 00:00:00 EST 2021
Design principles of the ESCRT-III Vps24-Vps2 module.
eLife
Banjade S,Shah YH,Tang S,Emr SD
459250 was used in Western Blot to allow us to propose three minimal features required for ESCRT-III function - spiral formation, lateral association of the spirals through heteropolymerization, and binding to the AAA + ATPase Vps4 for dynamic remodeling.
Mon May 24 00:00:00 EDT 2021
Target of Rapamycin Complex 1 (TORC1), Protein Kinase A (PKA) and Cytosolic pH Regulate a Transcriptional Circuit for Lipid Droplet Formation.
International journal of molecular sciences
Teixeira V,Martins TS,Prinz WA,Costa V
459250 was used in Western Blot to uncover the complex molecular relationships between the activity of major signaling effectors and lipid droplet accumulation.
Fri Aug 20 00:00:00 EDT 2021
Effects of A6E Mutation on Protein Expression and Supramolecular Assembly of Yeast Asparagine Synthetase.
Biology
Surasiang T,Noree C
459250 was used in Western Blot to test the effect of the human asparagine synthetase A6E mutation on yeast asparagine synthetase (Asn1/2p).
Sat Apr 03 00:00:00 EDT 2021
A genome-wide copper-sensitized screen identifies novel regulators of mitochondrial cytochrome c oxidase activity.
The Journal of biological chemistry
Garza NM,Griffin AT,Zulkifli M,Qiu C,Kaplan CD,Gohil VM
459250 was used in Western Blot to provide a mechanism by which vacuolar pH impacts mitochondrial respiration through copper homeostasis.
Thu Sep 09 00:00:00 EDT 2021
The histone chaperone Spt6 is required for normal recruitment of the capping enzyme Abd1 to transcribed regions.
The Journal of biological chemistry
Gopalakrishnan R,Winston F
459250 was used in Western Blot to characterize the differences in RNA polymerase II (RNAPII) interacting factors between wildtype cells and those depleted of the histone chaperone Spt6, leading to the identification of proteins that depend on Spt6 for their interaction with RNAPII.
Fri Oct 01 00:00:00 EDT 2021
The Cell Wall Integrity Receptor Mtl1 Contributes to Articulate Autophagic Responses When Glucose Availability Is Compromised.
Journal of fungi (Basel, Switzerland)
Montella-Manuel S,Pujol-Carrion N,de la Torre-Ruiz MA
459250 was used in Western Blot to demonstrate that Mtl1 plays a critical role in the detection of a descent in glucose concentration.
Tue Oct 26 00:00:00 EDT 2021
Characterization of HNRNPA1 mutations defines diversity in pathogenic mechanisms and clinical presentation.
JCI insight
Beijer D,Kim HJ,Guo L,O'Donovan K,Mademan I,Deconinck T,Van Schil K,Fare CM,Drake LE,Ford AF,Kochański A,Kabzińska D,Dubuisson N,Van den Bergh P,Voermans NC,Lemmers RJ,van der Maarel SM,Bonner D,Sampson JB,Wheeler MT,Mehrabyan A,Palmer S,De Jonghe P,Shorter J,Taylor JP,Baets J
459250 was used in Western Blot to characterize 4 potentially novel HNRNPA1 mutations and 2 known HNRNPA1 mutations previously connected to ALS and MSP, in a broad spectrum of patients with hereditary motor neuropathy, ALS, and myopathy.
Thu Jul 22 00:00:00 EDT 2021
Condensation of Ded1p Promotes a Translational Switch from Housekeeping to Stress Protein Production.
Cell
Iserman C,Desroches Altamirano C,Jegers C,Friedrich U,Zarin T,Fritsch AW,Mittasch M,Domingues A,Hersemann L,Jahnel M,Richter D,Guenther UP,Hentze MW,Moses AM,Hyman AA,Kramer G,Kreysing M,Franzmann TM,Alberti S
459250 was used in Western Blotting to demonstrate that, in budding yeast, the essential translation initiation factor Ded1p undergoes heatinduced phase separation into gel-like condensates.
Thu May 14 00:00:00 EDT 2020
A Prion Epigenetic Switch Establishes an Active Chromatin State.
Cell
Harvey ZH,Chakravarty AK,Futia RA,Jarosz DF
459250 was used in Western Blotting to study themechanism that facilitates inheritance of an activated domain of chromatin.
Thu Mar 05 00:00:00 EST 2020
The NADH Dehydrogenase Nde1 Executes Cell Death after Integrating Signals from Metabolism and Proteostasis on the Mitochondrial Surface.
Molecular cell
Saladi S,Boos F,Poglitsch M,Meyer H,Sommer F,Mühlhaus T,Schroda M,Schuldiner M,Madeo F,Herrmann JM
459250 was used in Western Blot to suggest that in addition to their role in electron transfer, mitochondrial NADH dehydrogenases such as Nde1 or AIF integrate signals from energy metabolism and cytosolic proteostasis to eliminate compromised cells from growing populations.
Thu Jan 02 00:00:00 EST 2020
Regulation of Cohesin-Mediated Chromosome Folding by Eco1 and Other Partners.
Molecular cell
Dauban L,Montagne R,Thierry A,Lazar-Stefanita L,Bastié N,Gadal O,Cournac A,Koszul R,Beckouët F
459250 was used in Western Blot to reveal that the mechanisms regulating cohesin-dependent chromatin loops are conserved among eukaryotes while promoting different functions.
Thu Mar 19 00:00:00 EDT 2020
Gene- and Species-Specific Hox mRNA Translation by Ribosome Expansion Segments.
Molecular cell
Leppek K,Fujii K,Quade N,Susanto TT,Boehringer D,Lenarčič T,Xue S,Genuth NR,Ban N,Barna M
459250 was used in Western Blot to show gene regulation directly mediated by rRNA and how ribosome evolution drives translation of critical developmental regulators.
Thu Dec 17 00:00:00 EST 2020
Liquidity Is a Critical Determinant for Selective Autophagy of Protein Condensates.
Molecular cell
Yamasaki A,Alam JM,Noshiro D,Hirata E,Fujioka Y,Suzuki K,Ohsumi Y,Noda NN
459250 was used in Western Blotting to show that the selective autophagic cargo aminopeptidase I (Ape1) undergoes phase separation to form semi-liquid droplets.
Thu Mar 19 00:00:00 EDT 2020
The conserved elongation factor Spn1 is required for normal transcription, histone modifications, and splicing in Saccharomyces cerevisiae.
Nucleic acids research
Reim NI,Chuang J,Jain D,Alver BH,Park PJ,Winston F
459250 was used in Western Blotting to show that Spn1 affects multiple aspects of gene expression and provide additional evidence that it functions as a histone chaperone in vivo.
Fri Oct 09 00:00:00 EDT 2020
An essential role for cardiolipin in the stability and function of the mitochondrial calcium uniporter.
Proceedings of the National Academy of Sciences of the United States of America
Ghosh S,Basu Ball W,Madaris TR,Srikantan S,Madesh M,Mootha VK,Gohil VM
459250 was used in Western Blotting to raise the hypothesis that impaired mitochondrial calcium transport contributes to the pathogenesis of Barth syndrome.
Tue Jul 14 00:00:00 EDT 2020
Homeostatic Control of Meiotic Prophase Checkpoint Function by Pch2 and Hop1.
Current biology : CB
Raina VB,Vader G
459250 was used in Western Blot to investigate the checkpoint roles of Pch2 and its connection to Hop1, a HORMA domain-containing client protein.
Mon Nov 16 00:00:00 EST 2020
The Structural Basis for Kinetochore Stabilization by Cnn1/CENP-T.
Current biology : CB
Hinshaw SM,Harrison SC
459250 was used in Western Blotting to present a high-resolution structure of the Ctf3c bound to the Cnn1-Wip1 heterodimer. Associated live-cell imaging experiments provide a mechanism for Ctf3c and Cnn1-Wip1 recruitment to the kinetochore.
Mon Sep 07 00:00:00 EDT 2020
The Phosphatase PP1 Promotes Mitotic Slippage through Mad3 Dephosphorylation.
Current biology : CB
Ruggiero A,Katou Y,Shirahige K,Séveno M,Piatti S
459250 was used in Western Blot to conclude that slippage is not the mere consequence of incomplete APC inactivation that brings about mitotic exit, as originally proposed, but involves the exertive antagonism between kinases and phosphatases.
Mon Jan 20 00:00:00 EST 2020
A Cycle of Ubiquitination Regulates Adaptor Function of the Nedd4-Family Ubiquitin Ligase Rsp5.
Current biology : CB
MacDonald C,Shields SB,Williams CA,Winistorfer S,Piper RC
459250 was used in Western Blotting to support a model whereby ubiquitinated Rsp5 adaptors are more active when "locked" onto Rsp5 via its N-lobe ubiquitin-binding surface and less active when they are "unlocked" by Ubp2-mediated deubiquitination.
Mon Feb 03 00:00:00 EST 2020
Calcineurin-dependent regulation of endocytosis by a plasma membrane ubiquitin ligase adaptor, Rcr1.
The Journal of cell biology
Zhu L,Sardana R,Jin DK,Emr SD
459250 was used in Western Blotting to show that two paralogous transmembrane Rsp5 adaptors, Rcr1 and Rcr2, are sorted to distinct cellular locations: Rcr1 is a plasma membrane (PM) protein, whereas Rcr2 is sorted to the vacuole.
Mon Aug 03 00:00:00 EDT 2020
Nuclear envelope-vacuole contacts mitigate nuclear pore complex assembly stress.
The Journal of cell biology
Lord CL,Wente SR
459250 was used in Western Blot to define how NE-vacuole interorganelle contacts coordinate responses to mitigate deleterious cellular effects caused by disrupted NPC assembly.
Mon Dec 07 00:00:00 EST 2020
Conserved metabolite regulation of stress granule assembly via AdoMet.
The Journal of cell biology
Begovich K,Vu AQ,Yeo G,Wilhelm JE
459250 was used in Western Blotting to provide evidence of a blood-based biomarker that may be used to monitor disease state in XL-CNM mice and patients and supports monitoring circulating myostatin during clinical trials for myotubular myopathy.
Mon Aug 03 00:00:00 EDT 2020
Conserved metabolite regulation of stress granule assembly via AdoMet.
The Journal of cell biology
Begovich K,Vu AQ,Yeo G,Wilhelm JE
459250 was used in Western Blotting to provide evidence of a blood-based biomarker that may be used to monitor disease state in XL-CNM mice and patients and supports monitoring circulating myostatin during clinical trials for myotubular myopathy.
Mon Aug 03 00:00:00 EDT 2020
The EB1-Kinesin-14 complex is required for efficient metaphase spindle assembly and kinetochore bi-orientation.
The Journal of cell biology
Kornakov N,Möllers B,Westermann S
459250 was used in Western Blot to show that key functions of budding yeast Kinesin-14 Cik1-Kar3 are accomplished in a complex with Bim1 (yeast EB1).
Mon Dec 07 00:00:00 EST 2020
Stable and destabilized GFP reporters to monitor calcineurin activity in Saccharomyces cerevisiae.
Microbial cell (Graz, Austria)
Diessl J,Nandy A,Schug C,Habernig L,Büttner S
459250 was used in Western Blot to generate stable and destabilized GFP transcriptional reporters under the control of a calcineurin-dependent response element (CDRE).
Wed Feb 05 00:00:00 EST 2020
PP2ACdc55 dephosphorylates Pds1 and inhibits spindle elongation in S. cerevisiae.
Journal of cell science
Khondker S,Kajjo S,Chandler-Brown D,Skotheim J,Rudner A,Ikui AE
459250 was used in Western Blotting to show that PP2ACdc55 directly dephosphorylates Pds1 both in vivo and in vitro Pds1 hyperphosphorylation in a cdc55 deletion mutant enhanced the Pds1-Esp1 interaction, which played a positive role in Pds1 nuclear accumulation and in spindle elongation.
Wed Jul 29 00:00:00 EDT 2020
A piggybacking mechanism enables peroxisomal localization of the glyoxylate cycle enzyme Mdh2 in yeast.
Journal of cell science
Gabay-Maskit S,Cruz-Zaragoza LD,Shai N,Eisenstein M,Bibi C,Cohen N,Hansen T,Yifrach E,Harpaz N,Belostotsky R,Schliebs W,Schuldiner M,Erdmann R,Zalckvar E
459250 was used in Western Blotting to how that the yeast malate dehydrogenase 2 (Mdh2) is dually localized to the cytosol and to peroxisomes and is targeted to peroxisomes via association with Mdh3 and a Pex5-dependent piggybacking mechanism.
Thu Dec 17 00:00:00 EST 2020
The Sts1 nuclear import adapter uses a non-canonical bipartite nuclear localization signal and is directly degraded by the proteasome.
Journal of cell science
Budenholzer L,Breckel C,Hickey CM,Hochstrasser M
459250 was used in Western Blotting to show that Sts1 contains a non-canonical bipartite nuclear localization signal (NLS) important for both nuclear localization of Sts1 itself and the proteasome.
Thu Mar 19 00:00:00 EDT 2020
Regulation of intrinsic polarity establishment by a differentiation-type MAPK pathway in S. cerevisiae.
Journal of cell science
Prabhakar A,Chow J,Siegel AJ,Cullen PJ
459250 was used in Western Blotting to show that the filamentous growth mitogen activated protein kinase (fMAPK) pathway regulates bud emergence under nutrient-limiting conditions.
Tue Apr 14 00:00:00 EDT 2020
Retrograde signaling mediates an adaptive survival response to endoplasmic reticulum stress in Saccharomyces cerevisiae.
Journal of cell science
Hijazi I,Knupp J,Chang A
459250 was used in Western Blotting to provide new insight into the role of respiration in cell survival in the face of ER stress, and should help in developing therapeutic strategies to limit cell death in disorders linked to ER stress.
Mon Mar 30 00:00:00 EDT 2020
Interactions of GMP with Human Glrx3 and with Saccharomyces cerevisiae Grx3 and Grx4 Converge in the Regulation of the Gcn2 Pathway.
Applied and environmental microbiology
Mechoud MA,Pujol-Carrion N,Montella-Manuel S,de la Torre-Ruiz MA
459250 was used in Western Blotting to show that the Glrx3/Grx3/Grx4 family presents an evolutionary and functional conservation in signaling events that is partly related to GMP function and contributes to cell life extension.
Thu Jul 02 00:00:00 EDT 2020
Damaging de novo missense variants in EEF1A2 lead to a developmental and degenerative epileptic-dyskinetic encephalopathy.
Human mutation
Carvill GL,Helbig KL,Myers CT,Scala M,Huether R,Lewis S,Kruer TN,Guida BS,Bakhtiari S,Sebe J,Tang S,Stickney H,Oktay SU,Bhandiwad AA,Ramsey K,Narayanan V,Feyma T,Rohena LO,Accogli A,Severino M,Hollingsworth G,Gill D,Depienne C,Nava C,Sadleir LG,Caruso PA,Lin AE,Jansen FE,Koeleman B,Brilstra E,Willemsen MH,Kleefstra T,Sa J,Mathieu ML,Perrin L,Lesca G,Striano P,Casari G,Scheffer IE,Raible D,Sattlegger E,Capra V,Padilla-Lopez S,Mefford HC,Kruer MC
459250 was used in Western Blot to confirm multiple variants as pathogenic and broaden the phenotypic spectrum to include dystonia/choreoathetosis, and in some cases a degenerative course with cerebral and cerebellar atrophy.
Wed Jul 01 00:00:00 EDT 2020
Deposition of Centromeric Histone H3 Variant CENP-A/Cse4 into Chromatin Is Facilitated by Its C-Terminal Sumoylation.
Genetics
Ohkuni K,Suva E,Au WC,Walker RL,Levy-Myers R,Meltzer PS,Baker RE,Basrai MA
459250 was used in Western Blotting to show that deposition of Cse4 into chromatin is facilitated by its C-terminal sumoylation.
Wed Apr 01 00:00:00 EDT 2020
Asymmetric Transcription Factor Partitioning During Yeast Cell Division Requires the FACT Chromatin Remodeler and Cell Cycle Progression.
Genetics
Herrero E,Stinus S,Bellows E,Berry LK,Wood H,Thorpe PH
459250 was used in Western Blotting to analyse the effects of core cellular processes in asymmetric cell division based on Ace2 localization.
Sun Nov 01 00:00:00 EDT 2020
The Yeast Hsp70 Cochaperone Ydj1 Regulates Functional Distinction of Ssa Hsp70s in the Hsp90 Chaperoning Pathway.
Genetics
Gaur D,Singh P,Guleria J,Gupta A,Kaur S,Sharma D
459250 was used in Western Blotting to reveal a novel role of Ydj1 in determining the functional distinction among Hsp70 isoforms with respect to the Hsp90 chaperoning action.
Wed Jul 01 00:00:00 EDT 2020
New Aspects of Invasive Growth Regulation Identified by Functional Profiling of MAPK Pathway Targets in Saccharomyces cerevisiae.
Genetics
Vandermeulen MD,Cullen PJ
459250 was used in Western Blotting to identify a new role for the pathway in negatively regulating invasive growth.
Tue Sep 01 00:00:00 EDT 2020
Plasma membrane tension regulates eisosome structure and function.
Molecular biology of the cell
Appadurai D,Gay L,Moharir A,Lang MJ,Duncan MC,Schmidt O,Teis D,Vu TN,Silva M,Jorgensen EM,Babst M
459250 was used in Western Blotting to demonstrate that eisosomes coordinate the regulation of both membrane tension and nutrient transporter stability.
Sat Feb 15 00:00:00 EST 2020
Functions for Cdc42p BEM adaptors in regulating a differentiation-type MAP kinase pathway.
Molecular biology of the cell
Basu S,González B,Li B,Kimble G,Kozminski KG,Cullen PJ
459250 was used in Western Blotting to study how Cdc42p regulates the fMAPK pathway through comparing Cdc42p-dependent MAPK pathways in the filamentous strain background.
Sun Mar 15 00:00:00 EDT 2020
Dysregulation of very-long-chain fatty acid metabolism causes membrane saturation and induction of the unfolded protein response.
Molecular biology of the cell
Micoogullari Y,Basu SS,Ang J,Weisshaar N,Schmitt ND,Abdelmoula WM,Lopez B,Agar JN,Agar N,Hanna J
459250 was used in Western Blotting to demonstrate that loss of the VLCFA-CoA synthetase Fat1, which is essential for VLCFA utilization, results in ER stress with compensatory UPR induction.
Wed Jan 01 00:00:00 EST 2020
The degradation-promoting roles of deubiquitinases Ubp6 and Ubp3 in cytosolic and ER protein quality control.
PloS one
Wu H,Ng DTW,Cheong I,Matsudaira P
459250 was used in Western Blotting to reveal that Ubp6 maintains the level of free ubiquitin to promote the elimination of misfolded cytosolic proteins, while Ubp3 supports the degradation of misfolded cytosolic and ER luminal proteins by different mechanisms.
Wed Jul 29 00:00:00 EDT 2020
Discovery of EMRE in fungi resolves the true evolutionary history of the mitochondrial calcium uniporter.
Nature communications
Pittis AA,Goh V,Cebrian-Serrano A,Wettmarshausen J,Perocchi F,Gabaldón T
459250 was used in Western Blotting to undertake a comprehensive evolutionary analysis of the mt-Ca2+ uniporter.
Wed Aug 12 00:00:00 EDT 2020
Tagging the proteasome active site β5 causes tag specific phenotypes in yeast.
Scientific reports
Waite KA,Burris A,Roelofs J
459250 was used in Western Blotting to shows that GFP-tag on the β5 subunit of the proteosome results in aberrant phenotypes that are not observed when several other CP subunits are tagged.
Thu Oct 22 00:00:00 EDT 2020
Poly(A)-Binding Protein Regulates the Efficiency of Translation Termination.
Cell reports
Wu C,Roy B,He F,Yan K,Jacobson A
459250 was used in Western Blotting to determine whether the relative position of a nonsense codon within an open reading frame influences termination efficiency.
Tue Nov 17 00:00:00 EST 2020
Pex14p Phosphorylation Modulates Import of Citrate Synthase 2 Into Peroxisomes in Saccharomyces cerevisiae.
Frontiers in cell and developmental biology
Schummer A,Maier R,Gabay-Maskit S,Hansen T,Mühlhäuser WWD,Suppanz I,Fadel A,Schuldiner M,Girzalsky W,Oeljeklaus S,Zalckvar E,Erdmann R,Warscheid B
459250 was used in Western Blotting to indicate that phosphorylation of Pex14p at S266 provides a mechanism for controlling the peroxisomal import of Cit2p, which helps S. cerevisiae cells to adjust their carbohydrate metabolism according to the nutritional conditions.
Tue Oct 13 00:00:00 EDT 2020
Aneuploidy-induced proteotoxic stress can be effectively tolerated without dosage compensation, genetic mutations, or stress responses.
BMC biology
Larrimore KE,Barattin-Voynova NS,Reid DW,Ng DTW
459250 was used in Western Blotting to show that yeast strains of some karyotypes in the genetic background studied here can tolerate the large aneuploidy-associated burden to the proteostasis machinery without genetic changes, dosage compensation, or activation of canonical stress response pathways.
Tue Sep 08 00:00:00 EDT 2020
Assigning mitochondrial localization of dual localized proteins using a yeast Bi-Genomic Mitochondrial-Split-GFP.
eLife
Bader G,Enkler L,Araiso Y,Hemmerle M,Binko K,Baranowska E,De Craene JO,Ruer-Laventie J,Pieters J,Tribouillard-Tanvier D,Senger B,di Rago JP,Friant S,Kucharczyk R,Becker HD
459250 was used in Western Blotting to engineer a yeast strain expressing a new type of Split-GFP that termed Bi-Genomic Mitochondrial-Split-GFP (BiG Mito-Split-GFP).
Mon Jul 13 00:00:00 EDT 2020
A Yeast Chromatin-enriched Fractions Purification Approach, yChEFs, from Saccharomyces cerevisiae.
Bio-protocol
Cuevas-Bermúdez A,Garrido-Godino AI,Gutiérrez-Santiago F,Navarro F
459250 was used in Western Blot to adapt a previous procedure and improve an approach named yChEFs (yeast Chromatin Enriched Fractions) for purifying chromatin fractions.
Sun Jan 05 00:00:00 EST 2020
Transcription-dependent enrichment of the yeast FACT complex influences nucleosome dynamics on the RNA polymerase III-transcribed genes.
RNA (New York, N.Y.)
Shukla A,Bhalla P,Potdar PK,Jampala P,Bhargava P
459250 was used in Western Blotting to show that Spt16 elicits a differential, gene-specific and stress-responsive dynamics, which provides a novel stress-sensor mechanism of regulating transcription against external stress.
Fri Dec 04 00:00:00 EST 2020
Therapeutic genetic variation revealed in diverse Hsp104 homologs.
eLife
March ZM,Sweeney K,Kim H,Yan X,Castellano LM,Jackrel ME,Lin J,Chuang E,Gomes E,Willicott CW,Michalska K,Jedrzejczak RP,Joachimiak A,Caldwell KA,Caldwell GA,Shalem O,Shorter J
459250 was used in Western Blotting to explore natural Hsp104 variation.
Tue Dec 15 00:00:00 EST 2020
Targeting Cdc42 with the anticancer compound MBQ-167 inhibits cell polarity and growth in the budding yeast S. cerevisiae.
Small GTPases
Rivera-Robles MJ,Medina-Velázquez J,Asencio-Torres GM,González-Crespo S,Rymond BC,Rodríguez-Medina J,Dharmawardhane S
459250 was used in Western Blotting to validate MBQ-167 as a Cdc42 inhibitor in another biological context and present a method to screen Cdc42 inhibitors with potential as anti-metastatic cancer drugs.
Sun Nov 01 00:00:00 EDT 2020
Cohesion is established during DNA replication utilising chromosome associated cohesin rings as well as those loaded de novo onto nascent DNAs.
eLife
Srinivasan M,Fumasoni M,Petela NJ,Murray A,Nasmyth KA
459250 was used in Western Blot to show here that in S. cerevisiae, both mechanisms exist and that each requires a different set of replisome-associated proteins.
Tue Jun 09 00:00:00 EDT 2020
Domain II of the translation elongation factor eEF1A is required for Gcn2 kinase inhibition.
FEBS letters
Ramesh R,Sattlegger E
459250 was used in Western Blotting to show that domain II of eEF1A plays an essential role in Gcn2 inhibition, while absence of domain III affects Gcn2 response pathways to impair eIF2α phosphorylation.
Wed Jul 01 00:00:00 EDT 2020
Peroxiredoxin promotes longevity and H2O2-resistance in yeast through redox-modulation of protein kinase A.
eLife
Roger F,Picazo C,Reiter W,Libiad M,Asami C,Hanzén S,Gao C,Lagniel G,Welkenhuysen N,Labarre J,Nyström T,Grøtli M,Hartl M,Toledano MB,Molin M
459250 was used in Immunoprecipitation to show that Tsa1 promotes resistance to H2O2 and extends lifespan upon caloric restriction by repressing the nutrient signalling Ras-cAMP-PKA pathway at the protein kinase A enzyme level in yeast.
Tue Jul 14 00:00:00 EDT 2020
Active transcription and Orc1 drive chromatin association of the AAA+ ATPase Pch2 during meiotic G2/prophase.
PLoS genetics
Cardoso da Silva R,Villar-Fernández MA,Vader G
459250 was used in Western Blotting to describe a comprehensive mapping of Pch2 localization across the budding yeast genome during meiotic G2/prophase.
Mon Jun 01 00:00:00 EDT 2020
Solid-phase inclusion as a mechanism for regulating unfolded proteins in the mitochondrial matrix.
Science advances
Ruan L,McNamara JT,Zhang X,Chang AC,Zhu J,Dong Y,Sun G,Peterson A,Na CH,Li R
459250 was used in Western Blotting to found that excessive unfolded proteins in the mitochondrial matrix of yeast cells are consolidated into solid-phase inclusions, which we term deposits of unfolded mitochondrial proteins (DUMP).
Sat Aug 01 00:00:00 EDT 2020
Association and dissociation between the mitochondrial Far complex and Atg32 regulate mitophagy.
eLife
Innokentev A,Furukawa K,Fukuda T,Saigusa T,Inoue K,Yamashita SI,Kanki T
459250 was used in Western Blotting to study whether Ppg1-mediated Far complex formation and its dissociation from Atg32 are crucial for mitophagy regulation.
Tue Dec 15 00:00:00 EST 2020
Mus81-Mms4 endonuclease is an Esc2-STUbL-Cullin8 mitotic substrate impacting on genome integrity.
Nature communications
Waizenegger A,Urulangodi M,Lehmann CP,Reyes TAC,Saugar I,Tercero JA,Szakal B,Branzei D
459250 was used in Western Blotting to highlight a replication protection function for Esc2-STUbL-Cul8 and emphasize the importance for genome stability of resetting phosphorylated Mms4 from one cycle to another.
Thu Nov 12 00:00:00 EST 2020
Chaperone-Mediated Protein Disaggregation Triggers Proteolytic Clearance of Intra-nuclear Protein Inclusions.
Cell reports
den Brave F,Cairo LV,Jagadeesan C,Ruger-Herreros C,Mogk A,Bukau B,Jentsch S
459250 was used in Western Blotting to demonstrate the coordinated activity of the Apj1/Hsp70 disaggregation system with the 26S proteasome in facilitating the clearance of toxic inclusions inside the nucleus.
Tue Jun 02 00:00:00 EDT 2020
The Rad53CHK1/CHK2-Spt21NPAT and Tel1ATM axes couple glucose tolerance to histone dosage and subtelomeric silencing.
Nature communications
Bruhn C,Ajazi A,Ferrari E,Lanz MC,Batrin R,Choudhary R,Walvekar A,Laxman S,Longhese MP,Fabre E,Smolka MB,Foiani M
459250 was used in Western Blotting to identify DDR mutations, histone imbalances and aberrant subtelomeric chromatin as interconnected causes of glucose dependence, implying that DDR kinases coordinate metabolism and epigenetic changes.
Wed Aug 19 00:00:00 EDT 2020
A Systematic Protein Turnover Map for Decoding Protein Degradation.
Cell reports
Christiano R,Arlt H,Kabatnik S,Mejhert N,Lai ZW,Farese RV,Walther TC
459250 was used in Western Blotting to present an analytical platform that combines systematic gene deletions with quantitative measures of protein turnover to deconvolve protein degradation pathways for Saccharomyces cerevisiae.
Tue Nov 10 00:00:00 EST 2020
Hsp90 Co-chaperones Form Plastic Genetic Networks Adapted to Client Maturation.
Cell reports
Biebl MM,Riedl M,Buchner J
459250 was used in Western Blot to analyze all double mutants of 11 Saccharomyces cerevisiae Hsp90 co-chaperones in vivo concerning effects on cell physiology and the activation of specific client proteins.
Tue Aug 25 00:00:00 EDT 2020
N-terminal protein acetylation by NatB modulates the levels of Nmnats, the NAD+ biosynthetic enzymes in Saccharomyces cerevisiae.
The Journal of biological chemistry
Croft T,Venkatakrishnan P,James Theoga Raj C,Groth B,Cater T,Salemi MR,Phinney B,Lin SJ
459250 was used in Western Blotting to examine whether NatB regulates NAD biosynthesis directly through Nt-acetylation of Nma1 and Nma2 and why the absence of Nt-acetylation causes the reduction of Nmnat protein levels in NatB mutants.
Fri May 22 00:00:00 EDT 2020
Complementary α-arrestin-ubiquitin ligase complexes control nutrient transporter endocytosis in response to amino acids.
eLife
Ivashov V,Zimmer J,Schwabl S,Kahlhofer J,Weys S,Gstir R,Jakschitz T,Kremser L,Bonn GK,Lindner H,Huber LA,Leon S,Schmidt O,Teis D
459250 was used in Western Blotting to show that amino acid excess or starvation activate complementary α-arrestin-Rsp5-complexes to control selective endocytosis and adapt nutrient acquisition.
Mon Aug 03 00:00:00 EDT 2020
The Cdc48 Complex Alleviates the Cytotoxicity of Misfolded Proteins by Regulating Ubiquitin Homeostasis.
Cell reports
Higgins R,Kabbaj MH,Sherwin D,Howell LA,Hatcher A,Tomko RJ,Wang Y
459250 was used in Western Blot to demonstrate that the Cdc48/p97 segregase machinery drives the clearance of ubiquitinated model misfolded protein Huntingtin (Htt103QP) and limits its aggregation.
Tue Jul 14 00:00:00 EDT 2020
Chaperone Function of Hgh1 in the Biogenesis of Eukaryotic Elongation Factor 2.
Molecular cell
Mönkemeyer L,Klaips CL,Balchin D,Körner R,Hartl FU,Bracher A
459250 was used in Western Blotting to show the highly conserved protein Hgh1 promotes efficient folding of the N-terminal GTPase module of eEF2.
Thu Apr 04 00:00:00 EDT 2019
The mRNA degradation factor Xrn1 regulates transcription elongation in parallel to Ccr4.
Nucleic acids research
Begley V,Corzo D,Jordán-Pla A,Cuevas-Bermúdez A,Miguel-Jiménez L,Pérez-Aguado D,Machuca-Ostos M,Navarro F,Chávez MJ,Pérez-Ortín JE,Chávez S
459250 was used in Western Blotting to perform accurate measurements of transcription and mRNA degradation in a set of mutants.
Thu Oct 10 00:00:00 EDT 2019
RNA surveillance by uridylation-dependent RNA decay in Schizosaccharomyces pombe.
Nucleic acids research
Chung CZ,Jaramillo JE,Ellis MJ,Bour DYN,Seidl LE,Jo DHS,Turk MA,Mann MR,Bi Y,Haniford DB,Duennwald ML,Heinemann IU
459250 was used in Western Blotting to investigate the prevalence and impact of uridylation-dependent RNA decay in S. pombe by transcriptionally profiling cid1 and dis3L2 deletion strains.
Mon Apr 08 00:00:00 EDT 2019
RNA polymerase II plays an active role in the formation of gene loops through the Rpb4 subunit.
Nucleic acids research
Allepuz-Fuster P,O'Brien MJ,González-Polo N,Pereira B,Dhoondia Z,Ansari A,Calvo O
459250 was used in Western Blotting to suggest RNA polymerase II subunit Rpb4 facilitates the TFIIB-Ssu72 interaction which is critical for gene loop formation.
Thu Sep 26 00:00:00 EDT 2019
Post-transcriptional negative feedback regulation of proteostasis through the Dis3 ribonuclease and its disruption by polyQ-expanded Huntingtin.
Nucleic acids research
Kong KE,Hung TF,Man PM,Wong TN,Cheng T,Jin DY
459250 was used in Western Blotting to uncover a mechanism that contributes to the impaired heat stress response in Huntington's disease.
Mon Nov 04 00:00:00 EST 2019
The Ulp2 SUMO protease promotes transcription elongation through regulation of histone sumoylation.
The EMBO journal
Ryu HY,Su D,Wilson-Eisele NR,Zhao D,López-Giráldez F,Hochstrasser M
459250 was used in Western Blotting to describe a novel chromatin regulatory mechanism whereby histone-H2B ubiquitylation stimulates histone sumoylation, which in turn appears to inhibit nucleosome association of the Ctk1 kinase.
Thu Aug 15 00:00:00 EDT 2019
Transfer of the Septin Ring to Cytokinetic Remnants in ER Stress Directs Age-Sensitive Cell-Cycle Re-entry.
Developmental cell
Chao JT,Piña F,Onishi M,Cohen Y,Lai YS,Schuldiner M,Niwa M
459250 was used in Western Blotting to investigate the mechanism of the septin ring regulation of the endoplasmic reticulum stress surveillance pathway.
Mon Oct 21 00:00:00 EDT 2019
Thiol-based direct threat sensing by the stress-activated protein kinase Hog1.
Science signaling
Guerra-Moreno A,Prado MA,Ang J,Schnell HM,Micoogullari Y,Paulo JA,Finley D,Gygi SP,Hanna J
459250 was used in Western Blotting to provide a mechanistic basis for understanding how stress-activated kinases can sense distinct threats and perform highly specific adaptive responses.
Tue Nov 26 00:00:00 EST 2019
COPII vesicles contribute to autophagosomal membranes.
The Journal of cell biology
Shima T,Kirisako H,Nakatogawa H
459250 was used in Western Blotting to identify the mechanism underlying autophagosome formation.
Mon May 06 00:00:00 EDT 2019
The RNA export factor Mex67 functions as a mobile nucleoporin.
The Journal of cell biology
Derrer CP,Mancini R,Vallotton P,Huet S,Weis K,Dultz E
459250 was used in Western Blot to use quantitative fluorescence microscopy techniques in live budding yeast cells to investigate how Mex67 facilitates mRNA export.
Mon Dec 02 00:00:00 EST 2019
Type I myosins anchor actin assembly to the plasma membrane during clathrin-mediated endocytosis.
The Journal of cell biology
Pedersen RTA,Drubin DG
459250 was used in Western Blotting to study type I myosins in actin assembly to the plasma membrane.
Mon Apr 01 00:00:00 EDT 2019
The mitochondrial phosphatidylserine decarboxylase Psd1 is involved in nitrogen starvation-induced mitophagy in yeast.
Journal of cell science
Vigié P,Cougouilles E,Bhatia-Kiššová I,Salin B,Blancard C,Camougrand N
459250 was used in Western Blotting to suggest that the mitophagy defect observed in δpsd1 yeast cells after nitrogen starvation may be due to a failure of Atg8 recruitment to mitochondria.
Wed Jan 02 00:00:00 EST 2019
Targeted Degradation of Glucose Transporters Protects against Arsenic Toxicity.
Molecular and cellular biology
Jochem M,Ende L,Isasa M,Ang J,Schnell H,Guerra-Moreno A,Micoogullari Y,Bhanu M,Gygi SP,Hanna J
459250 was used in Western Blotting to show resistance against arsenic toxicity can be achieved by degradation of glucose transporters indicating a major role of glucose transporters in arsenic import.
Wed May 15 00:00:00 EDT 2019
Regulation of the unfolded protein response in yeast by oxidative stress.
FEBS letters
Guerra-Moreno A,Ang J,Welsch H,Jochem M,Hanna J
459250 was used in Western Blotting to identify whether Ire1 within the unfolded protein response provides resistance to oxidative stress in yeast.
Wed May 01 00:00:00 EDT 2019
AMPK regulates ESCRT-dependent microautophagy of proteasomes concomitant with proteasome storage granule assembly during glucose starvation.
PLoS genetics
Li J,Breker M,Graham M,Schuldiner M,Hochstrasser M
459250 was used in Western Blotting to shows that direct uptake of proteasomes into the vacuole (lysosome) by microautophagy is a major means of proteasome elimination under limiting glucose conditions.
Fri Nov 01 00:00:00 EDT 2019
A systematic approach to identify recycling endocytic cargo depending on the GARP complex.
eLife
Eising S,Thiele L,Fröhlich F
459250 was used in Western Blotting to suggest that mis-sorting of flippases and remodeling of the lipid composition are the first occurring defects in GARP mutants.
Tue Jan 29 00:00:00 EST 2019
Endoplasmic reticulum stress differentially inhibits endoplasmic reticulum and inner nuclear membrane protein quality control degradation pathways.
The Journal of biological chemistry
Buchanan BW,Mehrtash AB,Broshar CL,Runnebohm AM,Snow BJ,Scanameo LN,Hochstrasser M,Rubenstein EM
459250 was used in Western Blot to investigate the impact of ER stress on ER and inner nuclear membrane protein quality control mechanisms in Saccharomyces cerevisiae.
Fri Dec 20 00:00:00 EST 2019
The endonuclease Cue2 cleaves mRNAs at stalled ribosomes during No Go Decay.
eLife
D'Orazio KN,Wu CC,Sinha N,Loll-Krippleber R,Brown GW,Green R
459250 was used in Western Blot to provide insights into how multiple decay processes converge to process problematic mRNAs in eukaryotic cells.
Thu Jun 20 00:00:00 EDT 2019
Candida albicans biofilm development is governed by cooperative attachment and adhesion maintenance proteins.
NPJ biofilms and microbiomes
McCall AD,Pathirana RU,Prabhakar A,Cullen PJ,Edgerton M
459250 was used in Western Blotting to analyse real-time imaging of C. albicans cells as they adhere and grow.
Wed Jun 24 00:00:00 EDT 2020
Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1.
eLife
Srinivasan M,Petela NJ,Scheinost JC,Collier J,Voulgaris M,B Roig M,Beckouët F,Hu B,Nasmyth KA
459250 was used in Western Blotting to show that cohesions rings that already have captured DNA via Scc2-dependent manner, no longer require Scc2 to capture sister DNAs.
Fri Jun 21 00:00:00 EDT 2019
The structure of the Ctf19c/CCAN from budding yeast.
eLife
Hinshaw SM,Harrison SC
459250 was used in Western Blotting to present the structure of a reconstituted 13-subunit Ctf19c determines by cryo-electron microscopy at ~4 Å resolution.
Thu Feb 14 00:00:00 EST 2019
DNA damage response activates respiration and thereby enlarges dNTP pools to promote cell survival in budding yeast.
The Journal of biological chemistry
Bu P,Nagar S,Bhagwat M,Kaur P,Shah A,Zeng J,Vancurova I,Vancura A
459250 was used in Western Blotting to study the effect of DNA damage response on the stimulation of respiration in yeast.
Fri Jun 21 00:00:00 EDT 2019
The mutational landscape of a prion-like domain.
Nature communications
Bolognesi B,Faure AJ,Seuma M,Schmiedel JM,Tartaglia GG,Lehner B
459250 was used in Western Blotting to reason that deep mutagenesis might be a powerful approach to disentangle the relationship between protein aggregation and toxicity, in relation to neurodegenerative diseases.
Fri Sep 13 00:00:00 EDT 2019
Polarisome scaffolder Spa2-mediated macromolecular condensation of Aip5 for actin polymerization.
Nature communications
Xie Y,Sun J,Han X,Turšić-Wunder A,Toh JDW,Hong W,Gao YG,Miao Y
459250 was used in Western Blotting to identify a previously functionally unknown polarisome member, actin-interacting-protein 5, which promotes actin assembly synergistically with formin Bni1.
Thu Nov 07 00:00:00 EST 2019
Structural insights into ubiquitin recognition and Ufd1 interaction of Npl4.
Nature communications
Sato Y,Tsuchiya H,Yamagata A,Okatsu K,Tanaka K,Saeki Y,Fukai S
459250 was used in Western Blotting to produce information about structural insight into the polyubiquitin chain recognition by the Cdc48-UN complex and its assembly.
Fri Dec 13 00:00:00 EST 2019
Dual control of NAD+ synthesis by purine metabolites in yeast.
eLife
Pinson B,Ceschin J,Saint-Marc C,Daignan-Fornier B
459250 was used in Western Blotting to study the mechanism underlying metabolism and biosynthesis in yeast.
Tue Mar 12 00:00:00 EDT 2019
The Hsp90 isoforms from S. cerevisiae differ in structure, function and client range.
Nature communications
Girstmair H,Tippel F,Lopez A,Tych K,Stein F,Haberkant P,Schmid PWN,Helm D,Rief M,Sattler M,Buchner J
459250 was used in Western Blotting to determine differences in the structure, function and interactomes of the two Hsp90 isoforms found in S. cerevisiae, and speculate that these differences allow adaptation to environmental challenges.
Fri Aug 09 00:00:00 EDT 2019
The Heat Shock Response in Yeast Maintains Protein Homeostasis by Chaperoning and Replenishing Proteins.
Cell reports
Mühlhofer M,Berchtold E,Stratil CG,Csaba G,Kunold E,Bach NC,Sieber SA,Haslbeck M,Zimmer R,Buchner J
459250 was used in Western Blot to support the concept that the HSR is modular with distinct programs dependent on the severity of the stress.
Tue Dec 24 00:00:00 EST 2019
The fitness cost and benefit of phase-separated protein deposits.
Molecular systems biology
Sanchez de Groot N,Torrent Burgas M,Ravarani CN,Trusina A,Ventura S,Babu MM
459250 was used in Western Blotting to show the selectable phenotypic variability, previously described for prions, could be a general property of proteins that can form phase-separated assemblies and may influence cell fitness.
Mon Apr 08 00:00:00 EDT 2019
Scc2 Is a Potent Activator of Cohesin's ATPase that Promotes Loading by Binding Scc1 without Pds5.
Molecular cell
Petela NJ,Gligoris TG,Metson J,Lee BG,Voulgaris M,Hu B,Kikuchi S,Chapard C,Chen W,Rajendra E,Srinivisan M,Yu H,Löwe J,Nasmyth KA
459250 was used in Western Blot to suggest that cohesin switches between two states: one with Pds5 bound that is unable to hydrolyze ATP efficiently but is capable of release from chromosomes and another in which Scc2 replaces Pds5 and stimulates ATP hydrolysis necessary for loading and translocation from loading sites.
Thu Jun 21 00:00:00 EDT 2018
Dot1 promotes H2B ubiquitination by a methyltransferase-independent mechanism.
Nucleic acids research
van Welsem T,Korthout T,Ekkebus R,Morais D,Molenaar TM,van Harten K,Poramba-Liyanage DW,Sun SM,Lenstra TL,Srivas R,Ideker T,Holstege FCP,van Attikum H,El Oualid F,Ovaa H,Stulemeijer IJE,Vlaming H,van Leeuwen F
459250 was used in Western Blotting to show that Dot1 and H2Bub1 are subject to bi-directional crosstalk and that Dot1 possesses chromatin regulatory functions that are independent of its methyltransferase activity.
Fri Nov 30 00:00:00 EST 2018
Phosphorylation-Mediated Clearance of Amyloid-like Assemblies in Meiosis.
Developmental cell
Carpenter K,Bell RB,Yunus J,Amon A,Berchowitz LE
459250 was used in Western Blotting to show amyloid-like assemblies are not as irreversible as once thought and can be cleared via phosphorylation in meiosis.
Mon May 07 00:00:00 EDT 2018
Regulated Crossing-Over Requires Inactivation of Yen1/GEN1 Resolvase during Meiotic Prophase I.
Developmental cell
Arter M,Hurtado-Nieves V,Oke A,Zhuge T,Wettstein R,Fung JC,Blanco MG,Matos J
459250 was used in Western Blotting to show that CDK-mediated phosphorylation of Yen1 averts its pervasive recruitment to recombination intermediates during prophase I.
Mon Jun 18 00:00:00 EDT 2018
Prolonged cyclin-dependent kinase inhibition results in septin perturbations during return to growth and mitosis.
The Journal of cell biology
Gihana GM,Musser TR,Thompson O,Lacefield S
459250 was used in Western Blotting to investigate how Saccharomyces cerevisiae coordinate polarisation, budding and anaphase during a unique developmental program called return to growth in which cells in meiosis return to mitosis upon nutrient shift.
Mon Jul 02 00:00:00 EDT 2018
The molecular recognition of phosphatidic acid by an amphipathic helix in Opi1.
The Journal of cell biology
Hofbauer HF,Gecht M,Fischer SC,Seybert A,Frangakis AS,Stelzer EHK,Covino R,Hummer G,Ernst R
459250 was used in Western Blotting to study the mechanism of membrane recognition by Opi1 and identify an amphipathic helix for selective binding of phosphatidic acid over phosphatidylserine.
Mon Sep 03 00:00:00 EDT 2018
Host Pah1p phosphatidate phosphatase limits viral replication by regulating phospholipid synthesis.
PLoS pathogens
Zhang Z,He G,Han GS,Zhang J,Catanzaro N,Diaz A,Wu Z,Carman GM,Xie L,Wang X
459250 was used in Western Blotting to show that Pah1p promotes storage lipid synthesis and thus represses phospholipid synthesis which in turn restricts both viral replication and cell growth during viral infection.
Sun Apr 01 00:00:00 EDT 2018
Swe1 and Mih1 regulate mitotic spindle dynamics in budding yeast via Bik1.
Journal of cell science
Raspelli E,Facchinetti S,Fraschini R
459250 was used in Western blotting to show that the kinase Swe1 and the phosphatase Mih1 are also involved in the control of mitotic spindle dynamics.
Mon Sep 03 00:00:00 EDT 2018
Hsp70-Hsp110 chaperones deliver ubiquitin-dependent and -independent substrates to the 26S proteasome for proteolysis in yeast.
Journal of cell science
Kandasamy G,Andréasson C
459250 was used in Western Blotting to investigate the mechanism by which Hsp110 interacts with Hsp70 at the 26S proteasome to facilitate protein degradation.
Tue Mar 20 00:00:00 EDT 2018
Lipid droplet consumption is functionally coupled to vacuole homeostasis independent of lipophagy.
Journal of cell science
Ouahoud S,Fiet MD,Martínez-Montañés F,Ejsing CS,Kuss O,Roden M,Markgraf DF
459250 was used in Western Blotting to investigate what genes are required for the functional coupling of Lipid droplets (LD) and other organelles during LD consumption by conducting a global screen in S. cerevisiae.
Mon Jun 11 00:00:00 EDT 2018
Adaptation to Endoplasmic Reticulum Stress Requires Transphosphorylation within the Activation Loop of Protein Kinases Kin1 and Kin2, Orthologs of Human Microtubule Affinity-Regulating Kinase.
Molecular and cellular biology
Ghosh C,Sathe L,Paprocki JD,Raicu V,Dey M
459250 was used in Western Blotting to show that during ER stress, the Kin kinase domain is released from its auto-inhibitory KA1 domain and is activated by trans-phosphorylation.
Sat Dec 01 00:00:00 EST 2018
Neurodegenerative Disease Proteinopathies Are Connected to Distinct Histone Post-translational Modification Landscapes.
ACS chemical neuroscience
Chen K,Bennett SA,Rana N,Yousuf H,Said M,Taaseen S,Mendo N,Meltser SM,Torrente MP
459250 was used in Western Blotting to delineate histone post-translational modification profiles in amyotrophic lateral sclerosis, Parkinson's disease and other neurodegenerative diseases.
Wed Apr 18 00:00:00 EDT 2018
MICU1 Confers Protection from MCU-Dependent Manganese Toxicity.
Cell reports
Wettmarshausen J,Goh V,Huang KT,Arduino DM,Tripathi U,Leimpek A,Cheng Y,Pittis AA,Gabaldón T,Mokranjac D,Hajnóczky G,Perocchi F
459250 was used in Western Blotting to dissect the interdependence between the pore-forming subunit MCU and the calcium-sensing regulator MICU1.
Tue Nov 06 00:00:00 EST 2018
Viral proteins as a potential driver of histone depletion in dinoflagellates.
Nature communications
Irwin NAT,Martin BJE,Young BP,Browne MJG,Flaus A,Loewen CJR,Keeling PJ,Howe LJ
459250 was used in Western Blotting to show that dinoflagellate-viral-nucleoprotein impairs growth and antagonises chromatin by localising to histone binding sites, displacing nucleosomes and impairing transcription.
Wed Apr 18 00:00:00 EDT 2018
Mitophagy in Yeast: A Screen of Mitophagy-Deficient Mutants.
Methods in molecular biology (Clifton, N.J.)
Furukawa K,Kanki T
459250 was used in Western Blotting to describe protocols to study mitophagy in yeast via a genome-wide screen for mitophagy-deficient mutants using fluorescence microscopy and immunoblotting.
Wed Jan 23 00:00:00 EST 2019
Tip60-mediated lipin 1 acetylation and ER translocation determine triacylglycerol synthesis rate.
Nature communications
Li TY,Song L,Sun Y,Li J,Yi C,Lam SM,Xu D,Zhou L,Li X,Yang Y,Zhang CS,Xie C,Huang X,Shui G,Lin SY,Reue K,Lin SC
459250 was used in Western Blotting to determine the mechanism by which signalling networks sense fatty acids and connect to the stimulation of lipid synthesis.
Tue May 15 00:00:00 EDT 2018
Failed mitochondrial import and impaired proteostasis trigger SUMOylation of mitochondrial proteins.
The Journal of biological chemistry
Paasch F,den Brave F,Psakhye I,Pfander B,Jentsch S
459250 was used in Western Blotting to identify a large set of structurally and functionally unrelated mitochondrial proteins as substrates of the SUMO pathway in yeast.
Fri Jan 12 00:00:00 EST 2018
Inhibiting Stearoyl-CoA Desaturase Ameliorates α-Synuclein Cytotoxicity.
Cell reports
Vincent BM,Tardiff DF,Piotrowski JS,Aron R,Lucas MC,Chung CY,Bacherman H,Chen Y,Pires M,Subramaniam R,Doshi DB,Sadlish H,Raja WK,Solís EJ,Khurana V,Le Bourdonnec B,Scannevin RH,Rhodes KJ
459250 was used in Western Blotting to identify a new therapeutic target for diseases caused by α-synuclein, a small lipid-binding protein that misfolds and aggregates in Parkinson's disease and other disorders.
Tue Dec 04 00:00:00 EST 2018
Inositol polyphosphates regulate and predict yeast pseudohyphal growth phenotypes.
PLoS genetics
Norman KL,Shively CA,De La Rocha AJ,Mutlu N,Basu S,Cullen PJ,Kumar A
459250 was used in Western blotting following immunoprecipitation to identify the presence of elevated 5PP-InsP5 levels relative to other inositol pyrophosphates as an in vivo marker of hyper-filamentous growth.
Fri Jun 01 00:00:00 EDT 2018
Identification of protein lysine methylation readers with a yeast three-hybrid approach.
Epigenetics & chromatin
Rawłuszko-Wieczorek AA,Knodel F,Tamas R,Dhayalan A,Jeltsch A
459250 was used in Western Blotting to develop a modifed yeast three-hybrid (Y3H) system able to identify methylation-dependent protein–protein interactions.
Thu Jan 25 00:00:00 EST 2018
Cowpea chlorotic mottle bromovirus replication proteins support template-selective RNA replication in Saccharomyces cerevisiae.
PloS one
Sibert BS,Navine AK,Pennington J,Wang X,Ahlquist P
459250 was used in Western Blotting to study the ability of CCMV to support spherule formation and RNA replication in yeast.
Mon May 20 00:00:00 EDT 2019
General decapping activators target different subsets of inefficiently translated mRNAs.
eLife
He F,Celik A,Wu C,Jacobson A
459250 was used in Western Blotting to determine the roles of Pat1, Lsm1, and Dhh1 in decapping of general mRNAs.
Thu Dec 06 00:00:00 EST 2018
Distinct adaptive mechanisms drive recovery from aneuploidy caused by loss of the Ulp2 SUMO protease.
Nature communications
Ryu HY,López-Giráldez F,Knight J,Hwang SS,Renner C,Kreft SG,Hochstrasser M
459250 was used in Western Blotting to reveal that aneuploidy allows rapid adaptation to Ulp2 loss, but long-term adaptation restores euploidy.
Fri Dec 21 00:00:00 EST 2018
Polyglutamine toxicity assays highlight the advantages of mScarlet for imaging in Saccharomyces cerevisiae.
F1000Research
Albakri MB,Jiang Y,Genereaux J,Lajoie P
459250 was used in Dot blot to determine the impact of the latest generation of red fluorescent proteins on their binding partner.
Tue Nov 19 00:00:00 EST 2019
Cytosolic Protein Vms1 Links Ribosome Quality Control to Mitochondrial and Cellular Homeostasis.
Cell
Izawa T,Park SH,Zhao L,Hartl FU,Neupert W
459250 was used in Western Blot to show that yeast cells continually produce faulty mitochondrial polypeptides that stall on the ribosome during translation but are imported into the mitochondria.
Thu Nov 02 00:00:00 EDT 2017
Slp1-Emp65: A Guardian Factor that Protects Folding Polypeptides from Promiscuous Degradation.
Cell
Zhang S,Xu C,Larrimore KE,Ng DTW
459250 was used in Western Blotting to show a conserved endoplasmic reticulum membrane protein complex of Slp1 and Emp65 bind unfolded proteins and mediate protection from degradation during the folding process.
Thu Oct 05 00:00:00 EDT 2017
Systematic Identification of MCU Modulators by Orthogonal Interspecies Chemical Screening.
Molecular cell
Arduino DM,Wettmarshausen J,Vais H,Navas-Navarro P,Cheng Y,Leimpek A,Ma Z,Delrio-Lorenzo A,Giordano A,Garcia-Perez C,Médard G,Kuster B,García-Sancho J,Mokranjac D,Foskett JK,Alonso MT,Perocchi F
459250 was used in Western Blot to introduce a high-throughput assay that selects for human MCU-specific small-molecule modulators in primary drug screens.
Thu Aug 17 00:00:00 EDT 2017
In Vivo Ubiquitin Linkage-type Analysis Reveals that the Cdc48-Rad23/Dsk2 Axis Contributes to K48-Linked Chain Specificity of the Proteasome.
Molecular cell
Tsuchiya H,Ohtake F,Arai N,Kaiho A,Yasuda S,Tanaka K,Saeki Y
459250 was used in Western Blot to investigate the ubiquitin network and analyse different ubiquitin binding domain proteins within yeast.
Thu May 18 00:00:00 EDT 2017
Upregulation of SPS100 gene expression by an antisense RNA via a switch of mRNA isoforms with different stabilities.
Nucleic acids research
Bunina D,Štefl M,Huber F,Khmelinskii A,Meurer M,Barry JD,Kats I,Kirrmaier D,Huber W,Knop M
459250 was used in Western Blotting to suggest a novel mechanism of antisense-dependent gene regulation via mRNA isoform switching.
Thu Nov 02 00:00:00 EDT 2017
Translational control of lipogenic enzymes in the cell cycle of synchronous, growing yeast cells.
The EMBO journal
Blank HM,Perez R,He C,Maitra N,Metz R,Hill J,Lin Y,Johnson CD,Bankaitis VA,Kennedy BK,Aramayo R,Polymenis M
459250 was used in western blot to identify a relationship between lipogenesis and protein synthesis in mitotic cell divisions
Wed Feb 15 00:00:00 EST 2017
Fundamental cell cycle kinases collaborate to ensure timely destruction of the synaptonemal complex during meiosis.
The EMBO journal
Argunhan B,Leung WK,Afshar N,Terentyev Y,Subramanian VV,Murayama Y,Hochwagen A,Iwasaki H,Tsubouchi T,Tsubouchi H
459250 was used in Western Blotting to propose that the concerted action of DDK, Polo-like kinase, and CDK1 promotes efficient SC destruction at the end of prophase I to ensure faithful inheritance of the genome.
Fri Sep 01 00:00:00 EDT 2017
Def1 interacts with TFIIH and modulates RNA polymerase II transcription.
Proceedings of the National Academy of Sciences of the United States of America
Damodaren N,Van Eeuwen T,Zamel J,Lin-Shiao E,Kalisman N,Murakami K
459250 was used in Western Blotting to extend the understanding of the molecular mechanism of transcription regulation on cellular stress and reveal functional similarities to the mammalian system.
Tue Dec 12 00:00:00 EST 2017
Histone H4 Facilitates the Proteolysis of the Budding Yeast CENP-ACse4 Centromeric Histone Variant.
Genetics
Deyter GM,Hildebrand EM,Barber AD,Biggins S
459250 was used in Western Blotting to show efficient histone variant CENP-ACse4 degradation requires a key residue in histone H4 that likely mediates an interaction between E3 ubiquitin ligase Psh1 and CENP-ACse4.
Sun Jan 01 00:00:00 EST 2017
Induction of proteotoxic stress by the mycotoxin patulin.
Toxicology letters
Guerra-Moreno A,Hanna J
459250 was used in Western Blotting to indicate that proteotoxicity represents a major aspect of patulin toxicity.
Wed Jul 05 00:00:00 EDT 2017
Chromatin-remodeling SWI/SNF complex regulates coenzyme Q6 synthesis and a metabolic shift to respiration in yeast.
The Journal of biological chemistry
Awad AM,Venkataramanan S,Nag A,Galivanche AR,Bradley MC,Neves LT,Douglass S,Clarke CF,Johnson TL
459250 was used in Western Blotting to reveal a link between Snf2 expression and the splicing of PTC7 and establish a role of SWI/SNF complex in the transition of yeast cells from fermentative to respiratory modes of metabolism.
Fri Sep 08 00:00:00 EDT 2017
Light-sensing via hydrogen peroxide and a peroxiredoxin.
Nature communications
Bodvard K,Peeters K,Roger F,Romanov N,Igbaria A,Welkenhuysen N,Palais G,Reiter W,Toledano MB,Käll M,Molin M
459250 was used in Western Blotting to identify a mechanism by which light could be sensed in all cells lacking dedicated photoreceptors.
Fri Mar 24 00:00:00 EDT 2017
Mitochondrial Cochaperone Mge1 Is Involved in Regulating Susceptibility to Fluconazole in Saccharomyces cerevisiae and Candida Species.
mBio
Demuyser L,Swinnen E,Fiori A,Herrera-Malaver B,Vestrepen K,Van Dijck P
459250 was used in Western Blotting to identify MGE1 as a multicopy suppressor of susceptibility to the antifungal fluconazole in the model yeast Saccharomyces cerevisiae and demonstrate that this phenomenon is not exclusively dependent on the integrity of the mitochondrial DNA or on the presence of the drug efflux pump Pdr5.
Tue Jul 18 00:00:00 EDT 2017
Determination of Cell Cycle Stage and Mitotic Exit Through the Quantification of the Protein Levels of Known Mitotic Regulators.
Methods in molecular biology (Clifton, N.J.)
Cepeda-García C
459250 was used in Western Blotting to describe the analysis of the protein levels of known mitotic regulators as molecular markers to monitor the progression of cells through the cell cycle by western blot in synchronized yeast cell cultures.
Fri Jan 19 00:00:00 EST 2018
Introduction of Premature Stop Codons as an Evolutionary Strategy To Rescue Signaling Network Function.
ACS synthetic biology
Kompella PS,Moses AM,Peisajovich SG
459250 was used in western blot to demonstrate that selection can compensate for protein overexpression by introducing premature stop codons
Fri Mar 17 00:00:00 EDT 2017
The unique C terminus of the calcineurin isoform CNAβ1 confers non-canonical regulation of enzyme activity by Ca2+ and calmodulin.
The Journal of biological chemistry
Bond R,Ly N,Cyert MS
459250 was used in Western Blotting to identify a distinct C-terminal autoinhibitory four-residue sequence in CNAΒ1, 462LAVP465, which competitively inhibits substrate dephosphorylation.
Fri Oct 06 00:00:00 EDT 2017
Failure of RQC machinery causes protein aggregation and proteotoxic stress.
Nature
Choe YJ,Park SH,Hassemer T,Körner R,Vincenz-Donnelly L,Hayer-Hartl M,Hartl FU
459250 was used in western blot to study the cause of protein aggregation and proteotoxic stress by failure of RQC machinery
Thu Mar 10 00:00:00 EST 2016
DNA damage signalling targets the kinetochore to promote chromatin mobility.
Nature cell biology
Strecker J,Gupta GD,Zhang W,Bashkurov M,Landry MC,Pelletier L,Durocher D
459250 was used in western blot to study how DNA damage signaling targets the kinetochore to induce chromatin mobility
Tue Mar 01 00:00:00 EST 2016
A new MCM modification cycle regulates DNA replication initiation.
Nature structural & molecular biology
Wei L,Zhao X
459250 was used in western blot to learn about regulation of DNA replication initiation caused by a new MCM modification cycle
Tue Mar 01 00:00:00 EST 2016
Engineering dynamical control of cell fate switching using synthetic phospho-regulons.
Proceedings of the National Academy of Sciences of the United States of America
Gordley RM,Williams RE,Bashor CJ,Toettcher JE,Yan S,Lim WA
459250 was used in western blot to design a modular approach for flexibly engineering phosphorylation circuits using designed phospho-regulon motifs
Tue Nov 22 00:00:00 EST 2016
An unrecognized function for COPII components in recruiting the viral replication protein BMV 1a to the perinuclear ER.
Journal of cell science
Li J,Fuchs S,Zhang J,Wellford S,Schuldiner M,Wang X
459250 was used in Western Blotting to show that a subset of COPII coat proteins have a role in targeting proteins to the perinuclear endoplasmic reticulum membrane.
Sat Oct 01 00:00:00 EDT 2016
Proteasome Impairment Induces Recovery of Mitochondrial Membrane Potential and an Alternative Pathway of Mitochondrial Fusion.
Molecular and cellular biology
Shirozu R,Yashiroda H,Murata S
459250 was used in western blot to investigate the role of the proteasome in the regulation of mitochondrial quality
Fri Jan 15 00:00:00 EST 2016
Soybean Ferritin Expression in Saccharomyces cerevisiae Modulates Iron Accumulation and Resistance to Elevated Iron Concentrations.
Applied and environmental microbiology
de Llanos R,Martínez-Garay CA,Fita-Torró J,Romero AM,Martínez-Pastor MT,Puig S
459250 was used in western blot to characterize modulation of iron accumulation and resistance to elevated iron concentrations due to soybean ferritin expression in Saccharomyces cerevisiae
Sun May 15 00:00:00 EDT 2016
Timely Closure of the Prospore Membrane Requires SPS1 and SPO77 in Saccharomyces cerevisiae.
Genetics
Paulissen SM,Slubowski CJ,Roesner JM,Huang LS
459250 was used in western blot to analyze the requirement for SPO77 and SPS1 in Saccharomyces cerevisiae and the timely closure of the prospore membrane
Fri Jul 01 00:00:00 EDT 2016
Dynamic relocation of the TORC1-Gtr1/2-Ego1/2/3 complex is regulated by Gtr1 and Gtr2.
Molecular biology of the cell
Kira S,Kumano Y,Ukai H,Takeda E,Matsuura A,Noda T
459250 was used in western blot to explore the TORCC1-Gtr1/2-Ego/1/2/3 complex and the genes that regulate it (Gtr1 and Gtr2)
Fri Jan 15 00:00:00 EST 2016
Incomplete proteasomal degradation of green fluorescent proteins in the context of tandem fluorescent protein timers.
Molecular biology of the cell
Khmelinskii A,Meurer M,Ho CT,Besenbeck B,Füller J,Lemberg MK,Bukau B,Mogk A,Knop M
459250 was used in western blot to research tandem fluorescent protein timers and incomplete proteasomal degradation of green fluorescent proteins
Fri Jan 15 00:00:00 EST 2016
A Concentration-Dependent Liquid Phase Separation Can Cause Toxicity upon Increased Protein Expression.
Cell reports
Bolognesi B,Lorenzo Gotor N,Dhar R,Cirillo D,Baldrighi M,Tartaglia GG,Lehner B
459250 was used in western blot describe a cellular toxicity mechanism based on a concentration-dependent liquid-liquid demixing
Tue Jun 28 00:00:00 EDT 2016
The Degradation Pathway of the Mitophagy Receptor Atg32 Is Re-Routed by a Posttranslational Modification.
PloS one
Levchenko M,Lorenzi I,Dudek J
459250 was used in western blot to discover that Atg32 undergoes a posttranslational modification upon induction of mitophagy
Mon Jul 03 00:00:00 EDT 2017
The ribosomal protein Asc1/RACK1 is required for efficient translation of short mRNAs.
eLife
Thompson MK,Rojas-Duran MF,Gangaramani P,Gilbert WV
459250 was used in western blot to analyze the requirement for efficient translation of short mRNAs of ribosomal protein Asc1/RACK1
Wed Apr 27 00:00:00 EDT 2016
Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae.
Journal of visualized experiments : JoVE
Buchanan BW,Lloyd ME,Engle SM,Rubenstein EM
459250 was used in western blot to study protein degradation in saccharomyces cerevisiae by cycloheximide chase analysis
Mon Apr 18 00:00:00 EDT 2016
The Atg1-kinase complex tethers Atg9-vesicles to initiate autophagy.
Nature communications
Rao Y,Perna MG,Hofmann B,Beier V,Wollert T
459250 was used in western blot to study initiation of autophagy by tethering Atg9-vesicles by the Atg1-kinase complex
Tue Jan 12 00:00:00 EST 2016
Regulation of Vacuolar H+-ATPase (V-ATPase) Reassembly by Glycolysis Flow in 6-Phosphofructo-1-kinase (PFK-1)-deficient Yeast Cells.
The Journal of biological chemistry
Chan CY,Dominguez D,Parra KJ
459250 was used in western blot to study how glycolysis flow in PFK-1 deficient yeast cells regulates V-ATPase reassembly
Fri Jul 22 00:00:00 EDT 2016
Mechanistic Insights into Hsp104 Potentiation.
The Journal of biological chemistry
Torrente MP,Chuang E,Noll MM,Jackrel ME,Go MS,Shorter J
459250 was used in western blot to investigate mechanisms of Hsp104 potentiation
Fri Mar 04 00:00:00 EST 2016
Phosphorylation of Yeast Pah1 Phosphatidate Phosphatase by Casein Kinase II Regulates Its Function in Lipid Metabolism.
The Journal of biological chemistry
Hsieh LS,Su WM,Han GS,Carman GM
459250 was used in western blot to analyze regulation of function in lipid metabolism by phosphorylation of Yeast Pah1 phosphatidate phosphatase by casein kinase II
Fri May 06 00:00:00 EDT 2016
Prioritized Expression of BTN2 of Saccharomyces cerevisiae under Pronounced Translation Repression Induced by Severe Ethanol Stress.
Frontiers in microbiology
Yamauchi Y,Izawa S
459250 was used in western blot to investigate the role of BTN2 in yeast's response to severe ethanol stress
Wed Sep 07 00:00:00 EDT 2016
Potassium and the K+/H+ Exchanger Kha1p Promote Binding of Copper to ApoFet3p Multi-copper Ferroxidase.
The Journal of biological chemistry
Wu X,Kim H,Seravalli J,Barycki JJ,Hart PJ,Gohara DW,Di Cera E,Jung WH,Kosman DJ,Lee J
459250 was used in western blot to analyze the promotion of binding of copper to ApoFet3p multi-copper ferroxidase by potassium and the K+/H+ exchanger Kha1p
Fri Apr 29 00:00:00 EDT 2016
Accessory Interaction Motifs in the Atg19 Cargo Receptor Enable Strong Binding to the Clustered Ubiquitin-related Atg8 Protein.
The Journal of biological chemistry
Abert C,Kontaxis G,Martens S
459250 was used in western blot clarify the interaction between Atg19 and Atg8
Fri Sep 02 00:00:00 EDT 2016
Scarless Gene Tagging with One-Step Transformation and Two-Step Selection in Saccharomyces cerevisiae and Schizosaccharomyces pombe.
PloS one
Landgraf D,Huh D,Hallacli E,Lindquist S
459250 was used in western blot to develop a scarless gene tagging method for yeast
Mon May 22 00:00:00 EDT 2017
Evolution-guided functional analyses reveal diverse antiviral specificities encoded by IFIT1 genes in mammals.
eLife
Daugherty MD,Schaller AM,Geballe AP,Malik HS
459250 was used in western blot to research diverse antiviral specificities encoded by IFIT1 genes in mammals by evolution-guided functional analyses
Tue May 31 00:00:00 EDT 2016
Starvation Induces Proteasome Autophagy with Different Pathways for Core and Regulatory Particles.
The Journal of biological chemistry
Waite KA,De-La Mota-Peynado A,Vontz G,Roelofs J
459250 was used in western blot to investigate degradation of the proteasome
Fri Feb 12 00:00:00 EST 2016
Structure and flexibility of the endosomal Vps34 complex reveals the basis of its function on membranes.
Science (New York, N.Y.)
Rostislavleva K,Soler N,Ohashi Y,Zhang L,Pardon E,Burke JE,Masson GR,Johnson C,Steyaert J,Ktistakis NT,Williams RL
459250 was used in immunocytochemistry to report the crystal structure of the 385-kilodalton endosomal complex II.
Fri Oct 09 00:00:00 EDT 2015
The coordinated action of the MVB pathway and autophagy ensures cell survival during starvation.
eLife
Müller M,Schmidt O,Angelova M,Faserl K,Weys S,Kremser L,Pfaffenwimmer T,Dalik T,Kraft C,Trajanoski Z,Lindner H,Teis D
459250 was used in western blot to study the interaction between the MVB pathway and autophagy ensures cell survival during starvation
Wed Apr 22 00:00:00 EDT 2015
One-step enzymatic modification of the cell surface redirects cellular cytotoxicity and parasite tropism.
ACS chemical biology
Swee LK,Lourido S,Bell GW,Ingram JR,Ploegh HL
459250 was used in western blot to describe a method to engineer the plasma membrane for research or therapy
Fri Feb 20 00:00:00 EST 2015
Regulation of Telomere Length Requires a Conserved N-Terminal Domain of Rif2 in Saccharomyces cerevisiae.
Genetics
Kaizer H,Connelly CJ,Bettridge K,Viggiani C,Greider CW
459250 was used in western blot to study how telomere length is regulated.
Thu Oct 01 00:00:00 EDT 2015
tRNA thiolation links translation to stress responses in Saccharomyces cerevisiae.
Molecular biology of the cell
Damon JR,Pincus D,Ploegh HL
459250 was used in western blot to analyze the link between translation and stress response in Saccharomyces cerevisiae by tRNA thiolation
Thu Jan 15 00:00:00 EST 2015
Growth-based determination and biochemical confirmation of genetic requirements for protein degradation in Saccharomyces cerevisiae.
Journal of visualized experiments : JoVE
Watts SG,Crowder JJ,Coffey SZ,Rubenstein EM
459250 was used in western blot to describe a new yeast growth-based assay for identifying genetic requirements needed for protein degradation
Mon Feb 16 00:00:00 EST 2015
Rio1 promotes rDNA stability and downregulates RNA polymerase I to ensure rDNA segregation.
Nature communications
Iacovella MG,Golfieri C,Massari LF,Busnelli S,Pagliuca C,Dal Maschio M,Infantino V,Visintin R,Mechtler K,Ferreira-Cerca S,De Wulf P
459250 was used in western blot to show that nuclear Rio1 integrates rDNA replication and segregation with ribosome biogenesis.
Wed Apr 08 00:00:00 EDT 2015
Quantitative Analysis of NF-κB Transactivation Specificity Using a Yeast-Based Functional Assay.
PloS one
Sharma V,Jordan JJ,Ciribilli Y,Resnick MA,Bisio A,Inga A
459250 was used in western blot to develop a NF-κB functional assay in yeast and study NF-κB functions, interactions, and chemical modulators.
Wed Mar 30 00:00:00 EDT 2016
Evaluating the activity of the filamentous growth mitogen-activated protein kinase pathway in yeast.
Cold Spring Harbor protocols
Cullen PJ
459250 was used in western blot to describe three assays to measure the activity of the filamentous growth pathway.
Mon Mar 02 00:00:00 EST 2015
On the Mechanism of Gene Silencing in Saccharomyces cerevisiae.
G3 (Bethesda, Md.)
Steakley DL,Rine J
459250 was used in western blot to investigate gene silencing in Saccharomyces cerevisiae.
Tue Jun 16 00:00:00 EDT 2015
Reciprocal conversion of Gtr1 and Gtr2 nucleotide-binding states by Npr2-Npr3 inactivates TORC1 and induces autophagy.
Autophagy
Kira S,Tabata K,Shirahama-Noda K,Nozoe A,Yoshimori T,Noda T
459250 was used in western blot to study the mechanisms that regulate autophagy
Mon Sep 01 00:00:00 EDT 2014
A cytosolic degradation pathway, prERAD, monitors pre-inserted secretory pathway proteins.
Journal of cell science
Ast T,Aviram N,Chuartzman SG,Schuldiner M
459250 was used in western blot to show that prERAD tags pre-inserted proteins that have remained on the cytosolic leaflet of the ER for too long for degradation.
Tue Jul 15 00:00:00 EDT 2014
Parkinson's disease-linked mutations in VPS35 induce dopaminergic neurodegeneration.
Human molecular genetics
Tsika E,Glauser L,Moser R,Fiser A,Daniel G,Sheerin UM,Lees A,Troncoso JC,Lewis PA,Bandopadhyay R,Schneider BL,Moore DJ
459250 was used in western blot to study the induction of dopaminergic neurodegeneration by VPS35 mutations associated with Parkinson's disease
Mon Sep 01 00:00:00 EDT 2014
Yeast Dun1 kinase regulates ribonucleotide reductase inhibitor Sml1 in response to iron deficiency.
Molecular and cellular biology
Sanvisens N,Romero AM,An X,Zhang C,de Llanos R,Martínez-Pastor MT,Bañó MC,Huang M,Puig S
459250 was used in western blot to demonstrate that the Dun1 checkpoint kinase promotes RNR function in response to Fe starvation by stimulating Sml1 protein degradation
Mon Sep 01 00:00:00 EDT 2014
Regulation of glucose-dependent gene expression by the RNA helicase Dbp2 in Saccharomyces cerevisiae.
Genetics
Beck ZT,Cloutier SC,Schipma MJ,Petell CJ,Ma WK,Tran EJ
459250 was used in western blot to investigate how Dbp2 integrates nutritional status and gene expression programs required for energy homeostasis
Sat Nov 01 00:00:00 EDT 2014
Semi-quantitative colony immunoassay for determining and optimizing protein expression in Saccharomyces cerevisiae and Escherichia coli.
Analytical biochemistry
Cridge AG,Visweswaraiah J,Ramesh R,Sattlegger E
459250 was used in western blot to describe a fast semi-quantitative colony immunoassay to detect intracellularly expressed proteins in both yeast and bacterial cells.
Sat Feb 15 00:00:00 EST 2014
Phosphorylation by casein kinase 2 facilitates Psh1 protein-assisted degradation of Cse4 protein.
The Journal of biological chemistry
Hewawasam GS,Mattingly M,Venkatesh S,Zhang Y,Florens L,Workman JL,Gerton JL
459250 was used in western blot to show that Psh1 is phosphorylated by the Cka2 subunit of casein kinase 2 and promotes its E3 activity for Cse4
Fri Oct 17 00:00:00 EDT 2014
Mlh2 is an accessory factor for DNA mismatch repair in Saccharomyces cerevisiae.
PLoS genetics
Campbell CS,Hombauer H,Srivatsan A,Bowen N,Gries K,Desai A,Putnam CD,Kolodner RD
459250 was used in western blot to study Saccharomyces cerevisiae and Mlh2 as an accessory factor for DNA mismatch repair
Thu May 01 00:00:00 EDT 2014
Compartmentalization of metabolic pathways in yeast mitochondria improves the production of branched-chain alcohols.
Nature biotechnology
Avalos JL,Fink GR,Stephanopoulos G
459250 was used in western blot to show that targeting metabolic pathways to mitochondria can increase production of compounds in yeast
Mon Apr 01 00:00:00 EDT 2013
Sequential primed kinases create a damage-responsive phosphodegron on Eco1.
Nature structural & molecular biology
Lyons NA,Fonslow BR,Diedrich JK,Yates JR,Morgan DO
459250 was used in western blot to identify proteins that regulate Eco1 degradation
Fri Feb 01 00:00:00 EST 2013
The lack of synchronization between iron uptake and cell growth leads to iron overload in Saccharomyces cerevisiae during post-exponential growth modes.
Biochemistry
Park J,McCormick SP,Chakrabarti M,Lindahl PA
459250 was used in western blot to study iron import and growth rates in yeast.
Tue Dec 31 00:00:00 EST 2013
Using yeast to uncover the regulation of protein kinase Cδ by ceramide.
FEMS yeast research
Bessa C,Pereira C,Leão M,Maciel C,Gomes S,Gonçalves J,Corte-Real M,Costa V,Saraiva L
459250 was used in western blot to assess the effect of ceramide on the activity of mammalian PKC isoforms.
Fri Nov 01 00:00:00 EDT 2013
Identification of new p53 target microRNAs by bioinformatics and functional analysis.
BMC cancer
Bisio A,De Sanctis V,Del Vescovo V,Denti MA,Jegga AG,Inga A,Ciribilli Y
459250 was used in western blot to review the roles and functions of p53.
Thu Nov 21 00:00:00 EST 2013
Human copper chaperone for superoxide dismutase 1 mediates its own oxidation-dependent import into mitochondria.
Nature communications
Suzuki Y,Ali M,Fischer M,Riemer J
459250 was used in western blot to elucidate how CCS is transported into mitochondria.
Tue Apr 01 00:00:00 EDT 2014
The exosome cofactor Rrp47 is critical for the stability and normal expression of its associated exoribonuclease Rrp6 in Saccharomyces cerevisiae.
PloS one
Feigenbutz M,Garland W,Turner M,Mitchell P
459250 was used in western blot to report that Rrp47 regulates expression levels of Rrp6 and that the Rrp6/Rrp47 complex and Rex1 are redundant for the 3' end maturation of box C/D snoRNAs.
Fri Jun 20 00:00:00 EDT 2014
Pex3-anchored Atg36 tags peroxisomes for degradation in Saccharomyces cerevisiae.
The EMBO journal
Motley AM,Nuttall JM,Hettema EH
459250 was used in Western Blot to conclude that Pex3 recruits the pexophagy receptor Atg36.
Fri Jun 29 00:00:00 EDT 2012
Secretory pathway-dependent localization of the Saccharomyces cerevisiae Rho GTPase-activating protein Rgd1p at growth sites.
Eukaryotic cell
Lefèbvre F,Prouzet-Mauléon V,Hugues M,Crouzet M,Vieillemard A,McCusker D,Thoraval D,Doignon F
459250 was used in western blot to suggest that secretory vesicles deliver RhoGAP Rgd1p to the bud tip and bud neck.
Tue May 01 00:00:00 EDT 2012
Clustering heterochromatin: Sir3 promotes telomere clustering independently of silencing in yeast.
The Journal of cell biology
Ruault M,De Meyer A,Loïodice I,Taddei A
459250 was used in western blot to investigate how telomeres cluster in perinuclear foci when associated with the Sir2-Sir3-Sir4 complex
Mon Feb 07 00:00:00 EST 2011
Intranuclear function for protein phosphatase 2A: Pph21 and Pph22 are required for rapamycin-induced GATA factor binding to the DAL5 promoter in yeast.
Molecular and cellular biology
Georis I,Tate JJ,Feller A,Cooper TG,Dubois E
459250 was used in western blot to study the role of Protein phosphatase 2A in intranuclear control of mRNA production
Sat Jan 01 00:00:00 EST 2011
Budding yeast Dma1 and Dma2 participate in regulation of Swe1 levels and localization.
Molecular biology of the cell
Raspelli E,Cassani C,Lucchini G,Fraschini R
459250 was used in western blot to elucidate how Dma1 and Dma2 regulate Swe1
Fri Jul 01 00:00:00 EDT 2011
Sumo-dependent substrate targeting of the SUMO protease Ulp1.
BMC biology
Elmore ZC,Donaher M,Matson BC,Murphy H,Westerbeck JW,Kerscher O
459250 was used in western blot to elucidate how Ulp1 is localized
Fri Oct 28 00:00:00 EDT 2011
Features of cryptic promoters and their varied reliance on bromodomain-containing factors.
PloS one
Pattenden SG,Gogol MM,Workman JL
459250 was used in western blot to investigate the role of histone modifications and co-activator recruitment in preinitiation complex formation
Thu Sep 23 00:00:00 EDT 2010
Noncanonical tandem SH2 enables interaction of elongation factor Spt6 with RNA polymerase II.
The Journal of biological chemistry
Diebold ML,Loeliger E,Koch M,Winston F,Cavarelli J,Romier C
459250 was used in western blot to report the structure of the Spt6 C-terminal region from Antonospora locustae
Fri Dec 03 00:00:00 EST 2010
Vta1p and Vps46p regulate the membrane association and ATPase activity of Vps4p at the yeast multivesicular body.
Proceedings of the National Academy of Sciences of the United States of America
Lottridge JM,Flannery AR,Vincelli JL,Stevens TH
459250 was used in western blot to discover roles for Vps46p and Vta1p in regulating the ESCRT complex assembly/disassembly cycle in protein sorting at the yeast late endosome
Tue Apr 18 00:00:00 EDT 2006