|Tested species reactivity||Human, Mouse, Non-human primate, Rat, Bovine|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant bovine p85 alpha subunit of PI-3 kinase, expressed in Baculovirus.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Internalization of prolactin receptor and prolactin in transfected cells does not involve nuclear translocation.
MA1-74183 was used in immunocytochemistry to investigate the mechanism for the internalization of prolactin and its receptor
|Perrot-Applanat M,Gualillo O,Buteau H,Edery M,Kelly PA||Journal of cell science (110 ( Pt 9):1123)||1997|
Null mutation of the prolactin receptor gene produces multiple reproductive defects in the mouse.
MA1-74183 was used in western blot to investigate the consequences of null mutation of the prolactin receptor with respect to reproduction in a mouse model
|Ormandy CJ,Camus A,Barra J,Damotte D,Lucas B,Buteau H,Edery M,Brousse N,Babinet C,Binart N,Kelly PA||Genes and development (11:167)||1997|
|Rat||10^7 cpm:50 ml||
Mutational analysis of the ligand-binding domain of the prolactin receptor.
MA1-74183 was used in western blot to perform mutational analysis of prolactin receptor in order to investigate the contributions of different domains to hormone binding
|Rozakis-Adcock M,Kelly PA||The Journal of biological chemistry (266:16472)||1991|
Purification and protein sequence analysis of rat liver prolactin receptor.
MA1-74183 was used in western blot to characterize prolactin receptors in rat liver
|Okamura H,Raguet S,Bell A,Gagnon J,Kelly PA||The Journal of biological chemistry (264:5904)||1989|
Mitogenic and binding properties of monoclonal antibodies to the prolactin receptor in Nb2 rat lymphoma cells. Selective enhancement by anti-mouse IgG.
MA1-74183 was used in blocking/activating experiment to perform functional analysis of monoclonal antibodies against prolactin receptor in Nb3 rat lymphoma cells
|Elberg G,Kelly PA,Djiane J,Binder L,Gertler A||The Journal of biological chemistry (265:14770)||1990|