|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Baculovirus expressed (recombinant) Bovine p85 alpha subunit of PI 3 Kinase.|
|Storage buffer||tissue culture supernatant diluted in 0.2M tris HCl, pH 7.4, with 5-10% FBS|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-21472 detects PI 3 Kinase p85 alpha in Human, Non-human primate, Mouse and Rat samples.
MA1-21472 has been successfully used in Immunoprecipitation and Western Blot procedures.
MA1-21472 immunogen corresponds to Baculovirus expressed (recombinant) Bovine p85 alpha subunit of PI 3 Kinase..
The enzyme PI-3-K phosphorylates at the D-3 position of the inositol ring of PI, PI-4- phosphate and PI-4,5-biphosphate (PI-4,5-P2) to produce their respective PI3-phosphorylated derivatives. PI-3-K exists as a heterodimer of 85 kDa (p85) and 110 kDa (p110) subunits. The p85 subunit contains two SH2 domains and an SH3 domain. It has been shown to associate with and serve as a substrate for activated growth factor receptor tyrosine kinases. p85 may serve as regulator of the catalytic subunit, p110, by acting as the link between PI-3-K and the ligand-activated receptor. Two distinct forms of the p85 subunit are described: p85 alpha, which binds tightly to the catalytic subunit and p85 beta, a protein of unknown function. Both isoforms bind to activated receptors and serve as tyrosine kinase substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.