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Invitrogen
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:1,000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:10-1:100 | - |
Immunocytochemistry (ICC/IF) |
1:50-1:100 | View 1 publication 1 publication |
Immunoprecipitation (IP) |
5 µg | - |
Immunohistochemistry (IHC) |
- | View 5 publications 5 publications |
Product Specifications | |
---|---|
Species Reactivity |
Bovine, Dog, Human, Mouse, Non-human primate, Rabbit, Rat |
Published species |
Mouse, Rabbit |
Host/Isotype |
Mouse / IgG2a |
Class |
Monoclonal |
Type |
Antibody |
Clone |
MC5 |
Immunogen |
Purified bovine brain Protein Kinase C alpha. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Protein A |
Storage buffer |
PBS with 30% glycerol, 1mg/mL BSA |
Contains |
0.05% sodium azide |
Storage conditions |
-20°C |
RRID |
AB_2536865 |
Western blot analysis of MA1-157 detects an ~80 kDa protein in human, bovine, non-human primate, mouse, rat, dog and rabbit cells. In Jurkat, rat brain and MDCK cell lysates, a weak unknown band at ~140 kDa is also detected. MA1-157 shows specificity to PKC alpha and is non-reactive to other PKC family members.
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: aging-associated gene 6; EC 2.7.11.13; FHC; FTH; FTHL6; kinase PKC-alpha; KPCA; MGC104426; PIG15; PKC III; PKC-A; PKC-alpha; PKC-III; PLIF; protein kinase C; Protein kinase C alpha type; protein kinase C, alpha; protein kinase c-alpha; unnamed protein product
Gene Aliases: AAG6; AI875142; PKC-alpha; PKCA; PKRCA; PRKACA; PRKCA
UniProt ID: (Bovine) P04409, (Rabbit) P10102, (Human) P17252, (Mouse) P20444
Entrez Gene ID: (Bovine) 282001, (Rabbit) 100037720, (Human) 5578, (Dog) 490904, (Mouse) 18750, (Rat) 24680
Molecular Function:
non-receptor serine/threonine protein kinase
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