Invitrogen

PP1 gamma Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 Published Figures

4 References

View all (18) PP1 gamma antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

PP1 gamma Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PP1 gamma antibody (Product # PA5-21671) at 1:200 dilution.

PP1 gamma Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PPP1CC (Product # PA5-21671) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

PP1 gamma Antibody in Western Blot (WB) — Western Blot using PP1 gamma Polyclonal Antibody (Product # PA5-21671). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PP1 gamma Polyclonal Antibody (Product # PA5-21671) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

PP1 gamma Antibody in Western Blot, Immunohistochemistry, Immunoprecipitation (WB, IHC, IP) — Published figure using PP1 gamma polyclonal antibody (Product # PA5-21671) in Western Blot, Immunohistochemistry and Immunoprecipitation

PP1 gamma Antibody — Figure 4 HCV Core protein interacts with PP1gamma and MYPT1 in PBMC blasts. Postnuclear lysates from Human PBMCs stimulated with PHA and cultured during 5 days with IL-2, were subjected to HCV Core protein pull down. Proteins pulled down by HCV Core coated magnetic beads (PBMC Core band) or by uncoated magnetic beads (PBMC beads band) were analyzed by Western blot with anti-human MYPT1 (A) and anti-human PP1gamma (B). Molecular mass, in kDa, is indicated on the side and band quantitation at the bottom for each Western blot.
{TM}

Product Details

PA5-21671

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunoprecipitation (IP)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 2 and 227 of PP1C gamma (Uniprot ID#P36873) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.79 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_11153939

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, Molt-4, Raji, NIH-3T3.

Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Dog (99%), Pig (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene belongs to the protein phosphatase family, PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. It is expressed in mammalian cells as three closely related isoforms, alpha, beta/delta and gamma, which have distinct localization patterns. This gene encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Ultrastructural Localization and Molecular Associations of HCV Capsid Protein in Jurkat T Cells.

Frontiers in microbiology

Fernández-Ponce C,Durán-Ruiz MC,Narbona-Sánchez I,Muñoz-Miranda JP,Arbulo-Echevarria MM,Serna-Sanz A,Baumann C,Litrán R,Aguado E,Bloch W,García-Cozar F

Published figure using PP1 gamma polyclonal antibody (Product # PA5-21671) in Western Blot

Wed Sep 30 00:00:00 EDT 2020

Cohesin Removal along the Chromosome Arms during the First Meiotic Division Depends on a NEK1-PP1γ-WAPL Axis in the Mouse.

Cell reports

Brieño-Enríquez MA,Moak SL,Toledo M,Filter JJ,Gray S,Barbero JL,Cohen PE,Holloway JK

PA521671 was used in western blot to elucidate how NEK1 regulates cohesin assembly on chromosomes at meiotic prophase I

Tue Oct 18 00:00:00 EDT 2016

Cohesin Removal along the Chromosome Arms during the First Meiotic Division Depends on a NEK1-PP1γ-WAPL Axis in the Mouse.

Cell reports

Brieño-Enríquez MA,Moak SL,Toledo M,Filter JJ,Gray S,Barbero JL,Cohen PE,Holloway JK

PA521671 was used in western blot to elucidate how NEK1 regulates cohesin assembly on chromosomes at meiotic prophase I

Tue Oct 18 00:00:00 EDT 2016

Cohesin Removal along the Chromosome Arms during the First Meiotic Division Depends on a NEK1-PP1γ-WAPL Axis in the Mouse.

Cell reports

Brieño-Enríquez MA,Moak SL,Toledo M,Filter JJ,Gray S,Barbero JL,Cohen PE,Holloway JK

PA521671 was used in western blot to elucidate how NEK1 regulates cohesin assembly on chromosomes at meiotic prophase I

Tue Oct 18 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: MGC1675; MGC2870; PP-1G; PP1C gamma 1; PP1C gamma 2; PP1G; PP1gamma; PP2CG; PP2CGAMMA; PPP2CG; protein phosphatase 1, catalytic subunit, gamma isoform; protein phosphatase 1, catalytic subunit, gamma isozyme; Protein phosphatase 1C catalytic subunit; serine/threonine phosphatase 1 gamma; Serine/threonine-protein phosphatase PP1-gamma catalytic subunit

Gene Aliases: dis2m1; PP-1G; PP1; PP1C; PPP1CC; PPP1G

UniProt ID: (Human) P36873, (Mouse) P63087

Entrez Gene ID: (Human) 5501, (Mouse) 19047

Molecular Function: protein phosphatase