Invitrogen

PRDM5 Monoclonal Antibody (7D4C12)

View all (7) PRDM5 antibodies

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PRDM5 Antibody in Immunofluorescence (IF) — Immunocytochemistry analysis of PRDM5 in MCF-7 cells (green). Sample was incubated with PRDM5 monoclonal antibody (Product # MA5-31861) using a dilution of 1:100-1:500 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .

PRDM5 Antibody in Western Blot (WB) — Western blot analysis of PRDM5 in HL-60 (1), NIH/3T3 (2), SW480 (3), and HEK293 (4) cell lysate. Samples were incubated with PRDM5 monoclonal antibody (Product # MA5-31861) using a dilution of 1:500-1:2000.

PRDM5 Antibody in Western Blot (WB) — Western blot analysis of PRDM5 in HEK293 (1) and PRDM5 cell lysate. Samples were incubated with PRDM5 monoclonal antibody (Product # MA5-31861) using a dilution of 1:500-1:2000.

PRDM5 Antibody in Western Blot (WB) — Western blot analysis of PRDM5 in human PRDM5 recombinant protein. Sample was incubated with PRDM5 monoclonal antibody (Product # MA5-31861) using a dilution of 1:500-1:2000.

PRDM5 Antibody in Flow Cytometry (Flow) — Flow cytometry of PRDM5 in HeLa cells (green). Sample was incubated with PRDM5 monoclonal antibody (Product # MA5-31861) using a dilution of 1:200-1:400 followed by negative control (red).

PRDM5 Antibody in ELISA (ELISA) — ELISA analysis of PRDM5 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with PRDM5 monoclonal antibody (Product # MA5-31861) using a dilution of 1:10,000.

Product Details

Tested Applications

Dilution

ELISA (ELISA)

1:10,000

Flow Cytometry (Flow)

1:200-1:400

Immunocytochemistry (ICC)

1:100-1:500

Immunofluorescence (IF)

1:100-1:500

Western Blot (WB)

1:500-1:2000

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

7D4C12

Immunogen

Purified recombinant fragment of human PRDM5 (AA: 1-100) expressed in E. coli.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Target Information

PRDM5, a novel member of PR domain protein family, contains a PR-domain at the-NH2 terminus followed by 16 zinc finger motifs. It is found to have a role in cell differentiation and tumorigenesis. Reports suggest PRDM5 causes G2/M arrest and apoptosis upon infection of tumor cells and has been found to be silenced in breast, ovarian and lung cancers, suggesting a potential clinical significance.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: BCS2; PFM2; PR domain containing 5; PR domain zinc finger protein 5; PR domain-containing protein 5

Gene Aliases: 4432417F03Rik; 6530401I24Rik; AI197291; BCS2; E130112L17Rik; PFM2; PRDM5

UniProt ID: (Human) Q9NQX1, (Mouse) Q9CXE0

Entrez Gene ID: (Human) 11107, (Mouse) 70779

negative regulation of transcription from RNA polymerase II promoter mitotic cell cycle transcription, DNA-templated histone deacetylation negative regulation of transcription, DNA-templated histone H3-K9 methylation regulation of transcription, DNA-templated transcription from RNA polymerase II promoter chromatin modification methylation

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding protein binding methyltransferase activity sequence-specific DNA binding transcription regulatory region DNA binding metal ion binding repressing transcription factor binding rRNA (adenine-N6,N6-)-dimethyltransferase activity transcription regulatory region sequence-specific DNA binding nucleic acid binding DNA binding protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity