|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human PRKAA1 expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/200 - 1/400|
|Immunofluorescence (IF)||1/200 - 1/1000|
|Immunohistochemistry (IHC)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA5-15815 targets PRKAA1 in FACS, IF, IHC, and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.
The MA5-15815 immunogen is purified recombinant fragment of human PRKAA1 expressed in E. Coli.
MA5-15815 detects PRKAA1 which has a predicted molecular weight of approximately 64kDa.
The protein belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Phosphorylation of ULK1 by AMPK regulates translocation of ULK1 to mitochondria and mitophagy.
MA5-15815 was used in western blot to elucidate the function of ULK1 activation and translocation in mycophagy
|Tian W,Li W,Chen Y,Yan Z,Huang X,Zhuang H,Zhong W,Chen Y,Wu W,Lin C,Chen H,Hou X,Zhang L,Sui S,Zhao B,Hu Z,Li L,Feng D||FEBS letters (589:1847)||2015|