Western blot analysis of PRMT1 in (lane 1) HEK-293T (lane 2) HeLa (lane 3) G361 (lane 4) NRK (lane 5) MDBK (lane 6) MDCK (lane 7) CHO (lane 8) 3T3 lysates. Samples were probed with a PRMT1 monoclonal antibody (Product # MA1-25468) at a dilution of 2µg/ml and developed using a Goat Anti-mouse IgG-Peroxidase and a chemiluminescent substrate.
|Tested species reactivity||Bovine, Dog, Hamster, Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Full length PRMT1.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25468 detects PRMT1 from human, mouse, rat, bovine, canine, hamster, and monkey samples.
MA1-25468 has been successfully used in immunoprecipitation, and Western blot procedures.
The MA1-25468 immunogen is full length PRMT1.
The PRMT1 gene encodes a protein arginine methyltransferase that functions as a histone methyltransferase specific for H4 (see MIM 602822).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.