MA1-754 detects presenilin 2 protein (PS2) from rat, mouse, human, and nonhuman primate samples. No cross-reactivity is seen with presenilin 1.
MA1-754 has successfully been used in immuno-fluorescence, immunocytochemistry, Western blot and ELISA procedures. By Western blot, this antibody detects an ~20 kDa protein representing PS2 CT (C-terminal fragment) and the ~45 kDa full-length PS2 from transfected COS-7 cells. In 4% paraformaldehyde fixed RAW cells, MA1-754 showed specific Golgi and ER labeling.
The MA1-754 immunogen is a synthetic peptide corresponding to residues C L(317) P Y D P E M E E D S Y D S F G E P(334) of human PS2.
Familial Alzheimer's disease is often characterized by an early (<60 years of age) and rapid deterioration of the central nervous system. The symptoms are caused by the abnormal buildup of senile plaques composed of the 42 residue amyloid-beta peptide. This peptide is the result of the amyloid precursor protein being cleaved by the presenilin-gamma-secretase complex. Recent studies have linked mutations in presenilin 1 (PS1) and presenilin 2 (PS2) to a rapid increase in plaque accumulation.
PS1 and PS2 are integral membrane proteins that contain 6-8 transmembrane domains and are predominantly localized within the endoplasmic reticulum and Golgi of neurons within the brain. Within the transmembrane regions of PS1 and PS2 there is over 60% homology, with the largest divergence found at the N-terminus and large loop region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AD3LP; AD4; AD5; Alzheimer disease 4; E5-1; Presenilin-2; Presenilin-2 CTF subunit; Presenilin-2 NTF subunit; PS-2; PS2; PSEN 2; STM-2; STM2
Gene Aliases: AD3L; AD4; Ad4h; ALG-3; Alg3; CMD1V; PS-2; PS2; PSEN2; PSNL2; STM2