Invitrogen

PTGES2 Monoclonal Antibody (OTI2C3)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (15) PTGES2 antibodies

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Datasheet

Protocols

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PTGES2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Prostaglandin E synthase 2 was performed using 70% confluent log phase BeWo cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PTGES2 Monoclonal Antibody (OTI2C3) (Product # MA5-26128) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766, 1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with Hoechst 33342 (Product # H1399). F-actin (Panel c: Red) was stained with Alexa Fluor™ Plus 647 Phalloidin (Product # A30107, 1:2000 dilution). Panel d represents the merged image showing perinuclear and cytoplasm localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 40X magnification in CellInsight CX7 LZR High-Content Screening (HCS) Platform (Product # CX7A1110LZR) and externally deconvoluted (D.Sage et al. / Methods 115 (2017) 28–41).

PTGES2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human colon tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a PTGES2 monoclonal antibody (Product # MA5-26128).

PTGES2 Antibody in Western Blot (WB) — Western blot was performed using Anti-PTGES2 Monoclonal Antibody (OTI2C3) (Product # MA5-26128) and a 33 kDa band corresponding to Prostaglandin E synthase 2 was observed across all cell lysates tested. Membrane enriched extracts (30 µg lysate) of SW480 (Lane 1), HCT 116 (Lane 2), HT-29 (Lane 3), A549 (Lane 4), Caco-2 (Lane 5) and LoVo (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX), 12 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

PTGES2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. SW480 cells were transfected with Prostaglandin E synthase 2 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-PTGES2 Monoclonal Antibody (OTI2C3) (Product # MA5-26128). {KD}

Product Details

MA5-26128

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-
Immunocytochemistry (ICC/IF)	1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	OTI2C3
Immunogen	Full length human recombinant protein of PTGES2 produced in HEK293T cell View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2725145

Target Information

The protein encoded by this gene is a membrane-associated prostaglandin E synthase, which catalyzes the conversion of prostaglandin H2 to prostaglandin E2. This protein also has been shown to activate the transcription regulated by a gamma-interferon-activated transcription element (GATE). Multiple transcript variants have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ14038; gamma-interferon-activated transcriptional element-binding factor 1; GATE-binding factor 1; membrane-associated prostaglandin E synthase 2; Membrane-associated prostaglandin E synthase-2; MGC11289; Microsomal prostaglandin E synthase 2; microsomal prostaglandin E synthase-2; mPGE synthase-2; mPGES-2; Prostaglandin E synthase 2; Prostaglandin E synthase 2 truncated form; Prostaglandin-H(2) E-isomerase; RP11-395P17.2

Gene Aliases: C9orf15; GBF-1; GBF1; mPGES-2; PGES2; PTGES2

UniProt ID: (Human) Q9H7Z7

Entrez Gene ID: (Human) 80142

Molecular Function: reductase

Function(s)

prostaglandin biosynthetic process cell redox homeostasis positive regulation of transcription, DNA-templated secretion oxidation-reduction process

Process(es)

DNA binding protein binding electron carrier activity protein disulfide oxidoreductase activity lyase activity heme binding glutathione binding prostaglandin-E synthase activity

PTGES2 Monoclonal Antibody (OTI2C3)

Product Details

MA5-26128

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

References (0)

Bioinformatics

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