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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: E(101) Q H L E S P V P S A P G A L A G(117)|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4277 detects alpha- and beta-PUMA from human samples.
PA1-4277 has been successfully used in Western blot procedures. By Western blot this antibody recognizes both forms of the PUMA protein found at ~20 kDa and ~36 kDa in transfected U20S cells.
PA1-4277 immunizing peptide corresponds to amino acid residues E(101) Q H L E S P V P S A P G A L A G(117) of the human PUMA-a form of the PUMA protein.
A novel gene PUMA (p53 upregulated modulator of apoptosis) is a target for activation by the p53 tumor-suppressor gene. p53 functions as a transcriptional activator, and influences p53-inducible genes that play a role in the induction of apoptosis in response to p53. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
p53 up-regulated modulator of apoptosis
BBC3; JFY-1; JFY1; PUMA