|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide made to residues 269-279 of the human and mouse PTCH protein.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:10-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:500|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Suggested positive control: antigen standard for PTCH1 (transient overexpression lysate).
Mutations of the human Patched tumor suppressor gene (PTCH) have been identified in individuals with the nevoid basal cell carcinoma syndrome (NBCCS) as well as in sporadic basal cell carcinomas and medulloblastomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Sonic hedgehog expression is disrupted following in ovo ethanol exposure during early chick eye development.
PA1-46222 was used in western blot to study the effects of ethanol exposure of the expression of sonic hedgehog during early development of the chick eye
|Brennan D,Giles S||Reproductive toxicology (Elmsford, N.Y.) (41:49)||2013|