|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant rat Peroxiredoxin-6.|
|Storage buffer||whole serum|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:1,000 - 1:4,000|
|Immunohistochemistry (Frozen) (IHC (F))||1:100-1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1,000 - 1:4,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-18277 detects Peroxiredoxin-6 from human, mouse, and rat samples.
PA1-18277 has been successfully used in immunohistochemistry (paraffin and frozen), Western blot, and ELISA procedures. Antigen retrieval has been used for immunohistochimstry applications but may not be necessary.
The PA1-18277 immunogen is recombinant rat Peroxiredoxin-6.
Reconstitute with 100 ul of distilled water. Centrifuge to remove any insoluble material.
Peroxiredoxin-6 is involved in redox regulation of the cell and reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. Peroxiredoxin-6 may also play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.