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Immunohistochemistry analysis of AKT/PKB [pT308] showing staining in the cytoplasm of paraffin-embedded mouse prostate tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a AKT/PKB [pT308] Rabbit Polyclonal Antibody (44602G) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human , Mouse|
|Published species reactivity||Mouse , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Akt1 that contains threonine 308. The sequence is conserved among multiple species including mouse and rat Akt1 and Akt2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Insulin signaling in type 2 diabetes: experimental and modeling analyses reveal mechanisms of insulin resistance in human adipocytes.
44-602G was used in western blot to propose that insulin resistance in expanded adipose tissue results from cell growth restriction to prevent cell necrosis.
|Brännmark C,Nyman E,Fagerholm S,Bergenholm L,Ekstrand EM,Cedersund G,Strålfors P||The Journal of biological chemistry (288:9867)||2013|
Reconstitution of PTEN activity by CK2 inhibitors and interference with the PI3-K/Akt cascade counteract the antiapoptotic effect of human stromal cells in chronic lymphocytic leukemia.
||Shehata M,Schnabl S,Demirtas D,Hilgarth M,Hubmann R,Ponath E,Badrnya S,Lehner C,Hoelbl A,Duechler M,Gaiger A,Zielinski C,Schwarzmeier JD,Jaeger U||Blood (116:2513)||2010|
Phosphoinositide3-kinase regulates actin polymerization during delayed phagocytosis of Helicobacter pylori.
||Allen LA,Allgood JA,Han X,Wittine LM||Journal of leukocyte biology (78:220)||2005|
PKB-ALPHA, PKB/Akt, PKB, Rac, CWS6, AKT, PKBalpha, RAC-ALPHA, PRKBA, RAC, Akt
PKB alpha, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase, protein kinase B alpha, proto-oncogene c-Akt, rac protein kinase alpha, AKT1 kinase, Akt1m protein, protein kinase B-alpha, proto-oncogene c-AKT, related to A and C kinases, AKT, C-AKT, EC 220.127.116.11, PKB, PKB-alpha, RAC, RAC-alpha serine/threonine kinase, kinase Akt1