Keep as concentrated solution.
Positive Control: MCF-7, PC-12, Rat2, NIH-3T3(5 ng/ml PDGF treatment for 15 min).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
The Akt family of serine/threonine protein kinases (also called protein kinase B/PKB) plays a key role in insulin signaling, cellular survival, and transformation. Akt1 is activated by phosphorylation on residues threonine 308 and Serine 473. Thr308, in the catalytic domain, is phosphorylated by the protein kinase PDK1, and Ser473, in the COOH-terminal regulatory domain, is phosphorylated by putative serine 473 kinase. To date, a couple of proteins have been shown to physically interact with PKB.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AKT1 kinase; Akt1m protein; PKB; PKB alpha; Protein kinase B; Protein kinase B alpha; protein kinase B-alpha; Proto-oncogene c-Akt; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; related to A and C kinases; Thymoma viral proto-oncogene
Gene Aliases: Akt; Akt1; PKB; PKB/Akt; PKBalpha; Rac
UniProt ID: (Mouse) P31750
Entrez Gene ID: (Mouse) 11651
Molecular Function: annexin calcium-binding protein calmodulin intracellular calcium-sensing protein kinase non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase