Western blot analysis of Phospho-ATF2 pThr52 in UACC903 melanoma cell line lysate (DMSO) using a Phospho-ATF2 pThr52 polyclonal antibody (Product # PA5-35391). Results show a band at ~72kDa. Immunolabeling is reduced by treatment of the lysate with the PKC catalytic inhibitor Go6850 (lane 1).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding THr52 of human ATF2 conjugated to KLH|
|Storage buffer||0.1M HEPES, pH 7.5, with 0.15M NaCl, 0.1mg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:250|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, chicken, mouse, non-human primate, rat and Xenopus based on 100% sequence homology.
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element, an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. The protein is also a histone acetyltransferase that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined.
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